Novel multicompartmental bile acid-based microcapsules for pancreatic β-cell transplantation
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In recent studies, we developed microcapsules suitable for transplantation of pancreatic β-cells in our type 1 diabetic animal model. In this study, we integrated the bile acid ursodeoxycholic acid (UDCA) into the microcapsules and evaluated the feasibility of pancreatic β-cell microencapsulation using the mouse pancreatic β-cell line, NIT-1. We tested the morphology, physico-chemical characteristics as well as the biological functions (viability, insulin production and bioenegetics) of the microcapsules in vitro. UDCA produced microcapsules with good morphology, better mechanical strength (p < 0.01), and reduced swelling properties (p < 0.01), but lower cell viability (p < 0.05) and cell count per microcapsule (p < 0.01). This is the first time biological functions of microencapsulated β-cells have been analyzed in real-time using the Seahorse XF Extracellular Flux Analyzer.
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Characterization of a novel bile acid-based delivery platform for microencapsulated pancreatic ß-cells.Mooranian, Armin; Negrulj, R.; Arfuso, Frank; Al-Salami, Hani (2014)Introduction: In a recent study, we confirmed good chemical and physical compatibility of microencapsulated pancreatic β-cells using a novel formulation of low viscosity sodium alginate (LVSA), Poly-L-Ornithine (PLO), and ...
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