Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers.
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Abstract Knowledge of genetic diversity in A. rabieiprovides different levels of information that are importantin the management of crop germplasm resources. Geneflow on a regional level indicates a significant potential riskfor the regional spread of novel alleles that might contributeto fungicide resistance or the breakdown of resistancegenes. Simple sequence repeat (SSR) and mating type(MAT) markers were used to determine the geneticstructure, and estimate genetic diversity and the prevalenceof mating types in 103 Ascochyta rabiei isolates from sevencounties in the Ilam and Kermanshah provinces of westernIran (Ilam, Aseman abad, Holaylan, Chardavol, Darehshahr, Gilangharb, and Sarpul). A set of 3 microsatelliteprimer pairs revealed a total of 75 alleles; the number ofalleles varied from 15 to 34 for each marker. A high level ofgenetic variability was observed among A. rabiei isolates inthe region. Genetic diversity was high (He=0.788) withinpopulations with corresponding high average gene flow andlow genetic distances between populations. The smallestgenetic distance was observed between isolates from Ilamand Chardavol. Both mating types were present in allpopulations, with the majority of the isolates belonging toMat1-1 (64%), but within populations the proportions ofeach mating type were not significantly different from50%. Results from this study will be useful in breedingfor Ascochyta blight-resistant cultivars and developingnecessary control measures.
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Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markersNourollahi, K.; Javannikhah, M.; Naghavi, M.; Lichtenzveig, Judith; Okhovat, S.; Oliver, Richard; Ellwood, Simon (2010)Knowledge of genetic diversity in A. rabiei provides different levels of information that are important in the management of crop germplasm resources. Gene flow on a regional level indicates a significant potential risk ...
van Leeuwen, Stephen J. (1997)An understanding of the role of genetic and ecological factors that influence demographic change is paramount for the conservation of plant populations. These genetic and ecological factors often act in concert to influence ...
Lichtenzveig, Judith; Winter, P.; Abbo, S.; Shtienberg, D.; Kaiser, W.; Kahl, G. (2002)A genetic map was developed for the ascomycete Didymella rabiei (Kovachevski) v. Arx (anamorph: Ascochyta rabiei Pass. Labr.), the causal agent of Ascochyta blight in chickpea (Cicer arietinum L.). The map was generated ...