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dc.contributor.authorTacar, O.
dc.contributor.authorIndumathy, S.
dc.contributor.authorTan, M.
dc.contributor.authorBaindur-Hudson, S.
dc.contributor.authorFriedhuber, A.
dc.contributor.authorDass, Crispin
dc.date.accessioned2018-02-01T05:20:30Z
dc.date.available2018-02-01T05:20:30Z
dc.date.created2018-02-01T04:49:16Z
dc.date.issued2014
dc.identifier.citationTacar, O. and Indumathy, S. and Tan, M. and Baindur-Hudson, S. and Friedhuber, A. and Dass, C. 2014. Cardiomyocyte apoptosis vs autophagy with prolonged doxorubicin treatment: Comparison with osteosarcoma cells. Journal of Pharmacy and Pharmacology.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/61928
dc.identifier.doi10.1111/jphp.12324
dc.description.abstract

© 2014 Royal Pharmaceutical Society. Objective: Doxorubicin (Dox) is a frontline chemotherapeutic against osteosarcoma (OS) that is plagued by side effects, particularly in the heart. The specific objective of this article is to investigate whether low-dose Dox treatment had pro-autophagic effects in cardiomyocytes as well as osteosarcoma cells. Methods: This study characterises apoptotic (Bax) and autophagic (Beclin-1) biomarker levels in human OS and cardiomyocyte cell lines as well as in various tissues when mice are exposed to low (1mg/kg, thrice weekly) and high (3mg/kg thrice weekly) dose Dox for a month. Key findings: There was a decrease in Bax and increase in Beclin-1 in cardiac tissue in the high-dose group. Dox decreased Beclin-1 in the skin and liver, with no clear indication in the stomach, small intestine and testis. At low Dox doses of 10 and 100nm in cardiomyocytes and OS cells, there is a pro-apoptotic effect, with a quicker response in the 100-nm condition, and a slower but steady increase of a pro-apoptotic response at the lower 10-nm dose. However, electron microscopy images revealed changes to human OS cells that resembled autophagy. Human prostate, breast and colorectal cells treated with 10-nm Dox showed ~ 40% reduction in cell viability after 24h. Conclusion: In culture, cells of both cardiomyocytes and OS revealed a predominant pro-apoptotic response at the expense of autophagy, although both seemed to be occurring in vivo.

dc.publisherJohn Wiley & Sons Ltd.
dc.titleCardiomyocyte apoptosis vs autophagy with prolonged doxorubicin treatment: Comparison with osteosarcoma cells
dc.typeJournal Article
dcterms.source.issn0022-3573
dcterms.source.titleJournal of Pharmacy and Pharmacology
curtin.departmentSchool of Pharmacy
curtin.accessStatusFulltext not available


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