Novel reassortant H9N2 viruses in pigeons and evidence for antigenic diversity of H9N2 viruses isolated from quails in Egypt
MetadataShow full item record
© 2017 The Authors. The endemicity of avian influenza viruses (AIVs) among Egyptian poultry represents a public health risk. Co-circulation of low pathogenic AIV H9N2 subtype with highly pathogenic AIV H5N1 subtype in Egyptian farms provides a possibility to generate novel reassortant viruses. Here, the genetic characteristics of surface glycoproteins of 59 Egyptian H9N2 viruses, isolated between 2013 and 2015, were analysed. To elucidate the potential of genetic reassortment, 10 H9N2 isolates were selected based on different avian hosts (chickens, ducks, pigeons and quails) and phylogenetic analyses of their full genome sequences were conducted. Additionally, we performed antigenic analysis to further investigate the antigenic evolution of H9N2 viruses isolated during 2011–2015. Different viral characteristics including receptor-binding affinity and drug resistance of representative Egyptian H9N2 viruses were further investigated. The surface glycoproteins of current Egyptian H9N2 viruses were closely related to viruses of the G1-like lineage isolated from Egypt. Several genetic markers that enhance virulence in poultry and transmission to humans were detected. Analysis of the full genome of 10 H9N2 isolates indicated that two pigeon isolates inherited five internal genes from Eurasian AIVs circulating in wild birds. Antigenic conservation of different Egyptian H9N2 isolates from chickens, pigeons and ducks was observed, whereas quail isolates showed antigenic drift. The Egyptian H9N2 viruses preferentially bound to the human-like receptor rather than to the avian-like receptor. Our results suggest that the endemic H9N2 viruses in Egypt contain elements that may favour avian-to-human transmission and thus represent a public health risk.
Showing items related by title, author, creator and subject.
Genetic and antigenic evolution of H9N2 avian influenza viruses circulating in Egypt between 2011 and 2013Kandeil, A.; El-Shesheny, R.; Maatouq, A.; Moatasim, Y.; Shehata, M.; Bagato, O.; Rubrum, A.; Shanmuganatham, K.; Webby, R.; Ali, Mohammed; Kayali, G. (2014)© 2014, Springer-Verlag Wien. Avian influenza virus subtype H9N2 has been circulating in the Middle East since the 1990s. For uncertain reasons, H9N2 was not detected in Egyptian farms until the end of 2010. Circulation ...
Genetic characterization of highly pathogenic avian influenza A H5N8 viruses isolated from wild birds in EgyptKandeil, A.; Kayed, A.; Moatasim, Y.; Webby, R.; McKenzie, P.; Kayali, G.; Ali, Mohammed (2017)Â© 2017 The Authors. A newly emerged H5N8 influenza virus was isolated from green-winged teal in Egypt during December 2016. In this study, we provide a detailed characterization of full genomes of Egyptian H5N8 viruses ...
Antigenic diversity and cross-reactivity of avian influenza H5N1 viruses in Egypt between 2006 and 2011El-Shesheny, R.; Kayali, G.; Kandeil, A.; Cai, Z.; Barakat, A.; Ghanim, H.; Ali, Mohammed (2012)Influenza epidemics are a major health concern worldwide. Highly pathogenic avian influenza (HPAI) H5N1 viruses in Egypt have been subject to rapid genetic and antigenic changes since the first outbreak in February 2006 ...