Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, june 2013
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We describe a novel spike pseudoparticle neutralisation assay (ppNT) for seroepidemiological studies on Middle East respiratory syndrome coronavirus (MERSCoV) and apply this assay together with conventional microneutralisation (MN) tests to investigate 1,343 human and 625 animal sera. The sera were collected in Egypt as a region adjacent to areas where MERS has been described, and in Hong Kong, China as a control region. Sera from dromedary camels had a high prevalence of antibody reactive to MERS-CoV by MERS NT (93.6%) and MERS ppNT (98.2%) assay. The antibody titres ranged up to 1,280 and higher in MN assays and 10,240 and higher in ppNT assays. No other investigated species had any antibody reactivity to MERS-CoV. While seropositivity does not exclude the possibility of infection with a closely related virus, our data highlight the need to attempt detection of MERSCoV or related coronaviruses in dromedary camels. The data show excellent correlation between the conventional MN assay and the novel ppNT assay. The newly developed ppNT assay does not require Biosafety Level 3 containment and is thus a relatively high-throughput assay, well suited for large-scale seroepidemiology studies which are needed to better understand the ecology and epidemiology of MERS-CoV.
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Seroepidemiology of middle east respiratory syndrome (MERS) coronavirus in Saudi Arabia (1993) and Australia (2014) and characterisation of assay specificityHemida, M.; Perera, R.; Al Jassim, R.; Kayali, G.; Siu, L.; Wang, P.; Chu, K.; Perlman, S.; Ali, Mohammed; Alnaeem, A.; Guan, Y.; Poon, L.; Saif, L.; Peiris, M. (2014)The pseudoparticle virus neutralisation test (ppNT) and a conventional microneutralisation (MN) assay are specific for detecting antibodies to Middle East respiratory syndrome coronavirus (MERS-CoV) when used in ...
Cross-sectional surveillance of middle east respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016Ali, Mohammed; El-Shesheny, R.; Kandeil, A.; Shehata, M.; Elsokary, B.; Gomaa, M.; Hassan, N.; El Sayed, A.; El-Taweel, A.; Sobhy, H.; Fasina, F.; Dauphin, G.; El Masry, I.; Wolde, A.; Daszak, P.; Miller, M.; VonDobschuetz, S.; Gardner, E.; Morzaria, S.; Lubroth, J.; Makonnen, Y. (2017)© 2017, European Centre for Disease Prevention and Control (ECDC). All rights reserved. A cross-sectional study was conducted in Egypt to determine the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) ...
Ali, Mohammed; Shehata, M.; Gomaa, M.; Kandeil, A.; El-Shesheny, R.; Kayed, A.; El-Taweel, A.; Atea, M.; Hassan, N.; Bagato, O.; Moatasim, Y.; Mahmoud, S.; Kutkat, O.; Maatouq, A.; Osman, A.; McKenzie, P.; Webby, R.; Kayali, G. (2017)© The Author(s) 2017. Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe human infections and dromedary camels are considered an intermediary host. The dynamics of natural infection in camels are not ...