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    New approaches to ancient DNA: using novel substrates to characterise DNA preservation and past biodiversity in warm-climate ecosystems

    Grealy Alicia 2017.pdf (69.45Mb)
    Access Status
    Open access
    Authors
    Grealy, Alicia Catherine
    Date
    2016
    Supervisor
    Prof. Michael Bunce
    Dr James Haile
    Type
    Thesis
    Award
    PhD
    
    Metadata
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    Faculty
    Science and Engineering
    School
    Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/51741
    Collection
    • Curtin Theses
    Abstract

    Retrieving ancient DNA (aDNA) from fossils in warm, tropical environments remains a challenge. This thesis describes the development and application of next generation sequencing technologies in the search for warm-climate aDNA. Methods to extract, enrich and sequence aDNA from fossil ‘bulk bone’ and avian eggshell are successfully explored from sites in Australia and Madagascar. Collectively, the research provides new insights into past biodiversity and evolutionary processes in climates not previously considered conducive to DNA preservation.

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