Comparison between real-time PCR and conventional PCR for detection of LPAI-H5N1 in different water types
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The current study aimed to compare between conventional reverse transcription PCR and real-time reverse transcription PCR for detection of LPAI-H5NL in different water types with different environmental conditions (temperature and pH). The experiment was conducted using sea water and Nile water adjusted at different temperatures 20 and 34°C and different pH 6.5, 7.0, 7.5, 8.0. The results of real-time reverse transcription PCR showed that the Low Pathogenic Avian Influenza H5Nl virus was stable for extended periods of time in different water types compared with conventional reverse transcription PCR. In general, reverse transcription PCR and real time reverse transcription PCR showed the same results for the persistence of the virus at pH=7.0 when the temperature was 20°C. By using two different techniques, the results revealed that the LPAI-H5Nl virus persisted in Nile water longer than in sea water. In conclution, real-time PCR is the most powerful tool for detection of Avian Influenza H5N1 virus in different water types.
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