Survival of LPAI H5N1 in water containing tilapia zillii
MetadataShow full item record
© 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved. The present study aimed to test the survival of LPAI H5N1 in water in the presence of Tilapia zillii fishes, the experiment was conducted using aquarium filled with 45 liters of Nile water and nine fishes of Tilapia zillii. The presence of the virus was tested in water samples by real-time RT-PCR. The viability and the titer of the virus in water samples extracted from aquarium were detected by Plaque infectivity assay and Heamagglutination assay, respectively. The results showed that, the viral RNA persisted 28 days in aquarium water. The CT values showed that the concentration of the virus seemed to be lowered by the time. The virus was survived in water containing fishes Tilapia zillii for 8 days by using plaque assay technique. In water containing Tilapia zillii fishes, the titer of LPAI-H5N1 virus was 512 and 64 HA at zero time and 4 days, respectively. In conclusion, prolonged persistence and infectivity of the virus in water might provide an evidence for the possibility of re-infection of poultry and human who might use or deal with this contaminated water for different purposes.
Showing items related by title, author, creator and subject.
Parsons, Miles James Gerard (2009)Techniques of single- and multi-beam active acoustics and the passive recording of fish vocalisations were employed to evaluate the benefits and limitations of each technique as a method for assessing and monitoring fish ...
Assessment of the health of the Swan-Canning river system using biochemical markers of exposure of fishWebb, Diane (2005)Most environmental studies concerning the environmental health of the Swan- Canning River system have focussed on nutrient inputs from both rural and urban catchments that are the cause of algal blooms. On occasions these ...
Generative processing of animated partial depictions fosters fish identification skills: Eye tracking evidenceBoucheix, J.; Lowe, Ric (2017)Â© 2017 Presses Universitaires de France, Departement des Revues. All rights reserved. This study addressed the issue of learning fish locomotion patterns, and was realized within an international broader collaboration: ...