Survival of LPAI H5N1 in water containing tilapia zillii
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© 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved. The present study aimed to test the survival of LPAI H5N1 in water in the presence of Tilapia zillii fishes, the experiment was conducted using aquarium filled with 45 liters of Nile water and nine fishes of Tilapia zillii. The presence of the virus was tested in water samples by real-time RT-PCR. The viability and the titer of the virus in water samples extracted from aquarium were detected by Plaque infectivity assay and Heamagglutination assay, respectively. The results showed that, the viral RNA persisted 28 days in aquarium water. The CT values showed that the concentration of the virus seemed to be lowered by the time. The virus was survived in water containing fishes Tilapia zillii for 8 days by using plaque assay technique. In water containing Tilapia zillii fishes, the titer of LPAI-H5N1 virus was 512 and 64 HA at zero time and 4 days, respectively. In conclusion, prolonged persistence and infectivity of the virus in water might provide an evidence for the possibility of re-infection of poultry and human who might use or deal with this contaminated water for different purposes.
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