Validation and Identification of Invasive Salmonella Serotypes in Sub-Saharan Africa by Multiplex Polymerase Chain Reaction
MetadataShow full item record
© 2016 The Author. Salmonella enterica serovar Typhi and nontyphoidal Salmonella (NTS) cause the majority of bloodstream infections in sub-Saharan Africa; however, serotyping is rarely performed. We validated a multiplex polymerase chain reaction (PCR) assay with the White-Kauffmann-Le Minor (WKLM) scheme of serotyping using 110 Salmonella isolates from blood cultures of febrile children in Ghana and applied the method in other Typhoid Fever Surveillance in Africa Program study sites. In Ghana, 47 (43%) S. Typhi, 36 (33%) Salmonella enterica serovar Typhimurium, 14 (13%) Salmonella enterica serovar Dublin, and 13 (12%) Salmonella enterica serovar Enteritidis were identified by both multiplex PCR and the WKLM scheme separately. Using the validated multiplex PCR assay, we identified 42 (66%) S. Typhi, 14 (22%) S. Typhimurium, 2 (3%) S. Dublin, 2 (3%) S. Enteritidis, and 4 (6%) other Salmonella species from the febrile patients in Burkina Faso, Guinea-Bissau, Madagascar, Senegal, and Tanzania. Application of this multiplex PCR assay in sub-Saharan Africa could advance the knowledge of serotype distribution of Salmonella.
Showing items related by title, author, creator and subject.
A Multicountry Molecular Analysis of Salmonella enterica Serovar Typhi with Reduced Susceptibility to Ciprofloxacin in Sub-Saharan AfricaAl-Emran, H.; Eibach, D.; Krumkamp, R.; Ali, Mohammed; Baker, S.; Biggs, H.; Bjerregaard-Andersen, M.; Breiman, R.; Clemens, J.; Crump, J.; Cruz Espinoza, L.; Deerin, J.; Dekker, D.; Gassama Sow, A.; Hertz, J.; Im, J.; Ibrango, S.; Von Kalckreuth, V.; Kabore, L.; Konings, F.; Løfberg, S.; Meyer, C.; Mintz, E.; Montgomery, J.; Olack, B.; Pak, G.; Panzner, U.; Park, S.; Razafindrabe, J.; Rabezanahary, H.; Rakotondrainiarivelo, J.; Rakotozandrindrainy, R.; Raminosoa, T.; Schütt-Gerowitt, H.; Sampo, E.; Soura, A.; Tall, A.; Warren, M.; Wierzba, T.; May, J.; Marks, F. (2016)© 2016 The Author. Background. Salmonella enterica serovar Typhi is a predominant cause of bloodstream infections in sub-Saharan Africa (SSA). Increasing numbers of S. Typhi with resistance to ciprofloxacin have been ...
Pectin and xyloglucan influence the attachment of Salmonella enterica and Listeria monocytogenes to bacterial cellulose-derived plant cell wall modelsTan, M.; Rahman, S.; Dykes, Gary (2016)Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose ...
Salmonella infections in Antarctic fauna and island populations of wildlife exposed to human activities in coastal areas of AustraliaIveson, J.; Shellam, G.; Bradshaw, S.; Smith, D.; Mackenzie, John; Mofflin, R. (2009)Salmonella infections in Antarctic wildlife were first reported in 1970 and in a search for evidence linking isolations with exposure to human activities, a comparison was made of serovars reported from marine fauna in ...