Optimal immobilization of ß -galactosidase onto -carrageenan gel beads using response surface methodology and its applications
MetadataShow full item record
ß-Galactosidase (ß-gal) was immobilized by covalent binding on novel -carrageenan gel beads activated by two-step method; the gel beads were soaked in polyethyleneimine followed by glutaraldehyde. 22 full-factorial central composite experiment designs were employed to optimize the conditions for the maximum enzyme loading efficiency. 11.443 U of enzyme/g gel beads was achieved by soaking 40 units of enzyme with the gel beads for eight hours. Immobilization process increased the pH from 4.5 to 5.5 and operational temperature from 50 to 55°C compared to the free enzyme. The apparent Km after immobilization was 61.6 mM compared to 22.9 mM for free enzyme. Maximum velocity Vmax was 131.2 mol·min-1 while it was 177.1 mol·min-1 for free enzyme. The full conversion experiment showed that the immobilized enzyme form is active as that of the free enzyme as both of them reached their maximum 100% relative hydrolysis at 4 h. The reusability test proved the durability of the -carrageenan beads loaded with ß-galactosidase for 20 cycles with retention of 60% of the immobilized enzyme activity to be more convenient for industrial uses. © 2014 Magdy M. Elnashar et al.
Showing items related by title, author, creator and subject.
Comparative studies of free and immobilized phytase, produced by Penicillium purpurogenu GE1, using grafted alginate/carrageenan beadsAwad, G.; Esawy, M.; El-Gammal, E.; Ahmed, H.; Elnashar, Magdy; Atwa, N. (2015)Aim: The aim of the study was to immobilize the phytase enzyme produced by Penicillium purpurogenu GE1 on grafted alginate/carrageenan beads and study the properties of the immobilized enzyme in comparison with free ones. ...
Elnashar, Magdy; Danial, E.; Awad, G. (2009)Inulinase has been extracted from Penicillium chrysogenum P36 and immobilized on a novel matrix of grafted biopolymer. The crude enzyme has been characterized in terms of specific activity, optimum temperature, and ...
Danial, E.; Elnashar, Magdy; Awad, G. (2010)Grafted alginate beads were prepared using the Encapsulator by two methods, the one-step and the two-step. The methods of grafting were characterized by thermal gravimetric analysis and infrared (IR). The lass transition ...