Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance
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Authors
Oliver, Richard
Roberts, I.
Harling, R.
Kenyon, L.
Punt, P.
Dingemanse, M.
Van Den Hondel, C.
Date
1987Type
Journal Article
Metadata
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OLIVER RP, ROBERTS IN, HARLING R, KENYON L, PUNT PJ, DINGEMANSE MA & VAN DEN HONDEL CAMJJ (1987) Transformation of Fulvia fulva, a fungal pathogen of tomato to Hygromycin B resistance. Current Genetics 12 231-233
Faculty
Department of Environmental & Agriculture
School of Agriculture and Environment
Faculty of Science and Engineering
Remarks
A copy of this item may be available from Professor Richard Oliver
Email: Richard.oliver@curtin.edu.au
Collection
Abstract
A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato.
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