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dc.contributor.authorGemene, K.
dc.contributor.authorBakker, Eric
dc.date.accessioned2017-01-30T11:19:50Z
dc.date.available2017-01-30T11:19:50Z
dc.date.created2009-02-19T18:01:56Z
dc.date.issued2009
dc.identifier.citationGemene, Kebede and Bakker, Eric. 2009. Flash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes. Analytical Biochemistry 386: pp. 276-281.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/10605
dc.identifier.doi10.1016/j.ab.2008.12.023
dc.description.abstract

We report here on a highly sensitive and rapid detection technique, multipulse flash chronopotentiometry, for the anticoagulant polyion heparin and its antidote protamine. The technique is based on a localized titration of the polyions at the surface of an appropriately formulated polymeric ion-selective membrane devoid of ion exchange properties to prohibit spontaneous extraction processes. A defined ion flux from the sample side to the membrane is induced electrochemically by applying a current pulse of appropriate amplitude and sign. The resulting depletion of the measured ions at the membrane surface gives rise to a characteristic limiting current or transition time and is observed as an inflection point in the resulting chronopotentiogram. The limiting current and the square root of the transition time are linear functions of the concentration of the polyion and yield sensitive and rapid analytical information attractive for clinical diagnostics applications. The polyion protamine is detected in 10-fold diluted blood samples in a matter of seconds via a cathodic current pulse. The utility of the technique for monitoring heparin/protamine titrations in physiological saline solutions is demonstrated.

dc.publisherElsevier
dc.titleFlash chronopotentiometric sensing of the polyions protamine and heparin at ion-selective membranes
dc.typeJournal Article
dcterms.source.volume386
dcterms.source.startPage276
dcterms.source.endPage281
dcterms.source.issn00032697
dcterms.source.titleAnalytical Biochemistry
curtin.note

The link to the journal’s home page is: http://www.elsevier.com/wps/find/journaldescription.cws_home/622781/description#description

curtin.note

Copyright © 2009 Elsevier B.V. All rights reserved

curtin.accessStatusOpen access
curtin.facultySchool of Engineering
curtin.facultyNanochemistry Research Institute (Research Institute)


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