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dc.contributor.authorTan, Kar-Chun
dc.contributor.authorFerguson-Hunt, M.
dc.contributor.authorRybak, K.
dc.contributor.authorWaters, O.
dc.contributor.authorStanley, W.
dc.contributor.authorBond, C.
dc.contributor.authorStukenbrock, E.
dc.contributor.authorFriesen, T.
dc.contributor.authorFaris, J.
dc.contributor.authorMcDonald, B.
dc.contributor.authorOliver, Richard
dc.date.accessioned2017-01-30T11:20:26Z
dc.date.available2017-01-30T11:20:26Z
dc.date.created2015-03-03T20:17:02Z
dc.date.issued2012
dc.identifier.citationTan, K. and Ferguson-Hunt, M. and Rybak, K. and Waters, O. and Stanley, W. and Bond, C. and Stukenbrock, E. et al. 2012. Quantitative variation in effector activity of ToxA isoforms from Stagonospora nodorum and Pyrenophora tritici-repentis. Molecular Plant - Microbe Interactions. 25 (4): pp. 515-522.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/10700
dc.identifier.doi10.1094/MPMI-10-11-0273
dc.description.abstract

ToxA is a proteinaceous necrotrophic effector produced by Stagonospora nodorum and Pyrenophora tritici-repentis. In this study, all eight mature isoforms of the ToxA protein were purified and compared. Circular dichroism spectra indicated that all isoforms were structurally intact and had indistinguishable secondary structural features. ToxA isoforms were infiltrated into wheat lines that carry the sensitivity gene Tsn1. It was observed that different wheat lines carrying identical Tsn1 alleles varied in sensitivity to ToxA. All ToxA isoforms induced necrosis when introduced into any Tsn1 wheat line but we observed quantitative variation in effector activity, with the least-active version found in isolates of P. tritici-repentis. Pathogen sporulation increased with higher doses of ToxA. The isoforms that induced the most rapid necrosis also induced the most sporulation, indicating that pathogen fitness is affected by differences in ToxA activity. We show that differences in toxin activity encoded by a single gene can contribute to the quantitative inheritance of necrotrophic virulence. Our findings support the hypothesis that the variation at ToxA results from selection that favors increased toxin activity.

dc.publisherAMER PHYTOPATHOLOGICAL SOC
dc.titleQuantitative variation in effector activity of ToxA isoforms from Stagonospora nodorum and Pyrenophora tritici-repentis
dc.typeJournal Article
dcterms.source.volume25
dcterms.source.number4
dcterms.source.startPage515
dcterms.source.endPage522
dcterms.source.issn08940282
dcterms.source.titleMolecular Plant - Microbe Interactions
curtin.departmentDepartment of Environment and Agriculture
curtin.accessStatusFulltext not available


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