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    Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing

    Access Status
    Fulltext not available
    Authors
    Rathod, D.
    Warren, S.
    Keane, Kevin
    Egan, D.
    Dempsey, E.
    Date
    2011
    Type
    Journal Article
    
    Metadata
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    Citation
    Rathod, D. and Warren, S. and Keane, K. and Egan, D. and Dempsey, E. 2011. Evaluation of a modified carbon micromesh electrode as a new substrate for electrochemical immunosensing. Analytical Methods. 3 (4): pp. 799-805.
    Source Title
    Analytical Methods
    DOI
    10.1039/c0ay00665c
    ISSN
    1759-9660
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/10709
    Collection
    • Curtin Research Publications
    Abstract

    Here we report a novel immunosensor platform technology based on self-assembled monolayer (SAM) of electrodeposited gold (Au) nanostructures with subsequent antibody attachment. Carbon cloth micro-fibres were employed for the first time as a working electrode in an immunosensor device. Uniform distribution of Au nanoparticles was achieved on the surface of microfibers with regulation of particle size and shape made possible via controlled scan rate during deposition from aqueous AuCl4. The electrodes were successfully characterised using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA). Chemiluminescence detection confirmed successful attachment of anti-actin antibodies onto the modified conducting surface, allowing detection of various concentrations of the actin antigen (0.1-1 g ml-1). The binding of antigen to antibody was successfully measured using EIS in 1 mM K3Fe(CN)6/K 4Fe(CN)6 as a redox probe, which allowed determination of charge transfer resistance (Rct) changes upon binding. A linear increase in Rct was observed with increasing concentration of antigen (actin). Therefore, we have clearly demonstrated that this new electrochemical immunosensor design is effective, and can be applied to the analysis of a clinically significant protein. © 2011 The Royal Society of Chemistry.

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