Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Isolation, biochemical and molecular identification, and in-vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India

    Access Status
    Open access via publisher
    Authors
    Preethirani, P.
    Isloor, S.
    Sudareshan, S.
    Nuthanalakshmi, V.
    Deepthikiran, K.
    sinha, A.
    Rathnamma, D.
    Prabhu, K.
    Sharada, R.
    Mukkur, Trilochan
    Hegde, N.
    Date
    2015
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Preethirani, P. and Isloor, S. and Sudareshan, S. and Nuthanalakshmi, V. and Deepthikiran, K. and sinha, A. and Rathnamma, D. et al. 2015. Isolation, biochemical and molecular identification, and in-vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India. PLoS One. 10 (11): pp. 1-15.
    Source Title
    PLoS One
    DOI
    10.1371/journal.pone.0142717
    ISSN
    1932-6203
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/11128
    Collection
    • Curtin Research Publications
    Abstract

    Buffaloes are the second largest source of milk. Mastitis is a major impediment for milk production, but not much information is available about bubaline mastitis, especially subclinical mastitis. The aim of this study was to (a) investigate the application of various tests for the diagnosis of bubaline subclinical mastitis, (b) identify the major bacteria associated with it, and (c) evaluate the antibiotic resistance pattern of the bacteria. To this end, 190 quarter milk samples were collected from 57 domesticated dairy buffaloes from organized (64 samples) and unorganized (126 samples) sectors. Of these, 48.4%, 40.0%, 45.8%, 61.1%, and 61.6% were positive for subclinical mastitis by somatic cell count, electrical conductivity, California mastitis test, bromothymol blue test, and N-acetyl glucosaminidase test, respectively. As compared to the gold standard of somatic cell count, California mastitis test performed the best. However, a combination of the two methods was found to be the best option. Microbiological evaluation, both by biochemical methods as well as by monoplex and multiplex polymerase chain reaction, revealed that coagulase-negative staphylococci were the most predominant (64.8%) bacteria, followed by streptococci (18.1%), Escherichia coli (9.8%) and Staphylococcus aureus (7.3%). Most of the pathogens were resistant to multiple antibiotics, especially to β-lactam antibiotics. We propose that California mastitis test be combined with somatic cell count for diagnosis of subclinical mastitis in domestic dairy buffaloes. Further, our results reveal high resistance of the associated bacteria to the β-lactam class of antibiotics, and a possible major role of coagulase-negative staphylococci in causing the disease in India.

    Related items

    Showing items related by title, author, creator and subject.

    • Antimicrobial resistance in gram-positive cocci isolated from poultry in Western Australia : an assessment of poultry meat as a vehicle for the transmission of resistant strains via the food chain.
      Bertolatti, Dean (2002)
      The aim of this study was to examine whether Gram-positive cocci isolated from processed poultry in Western Australia provided a potential risk for the transfer of antimicrobial-resistant organisms to humans via commercially ...
    • Molecular analysis of genes encoding resistance to Cationic Biocides in staphylococci
      Morgan, Dale (2007)
      Bacterial resistance to non-antibiotic agents is being increasingly studied. Plasmid-mediated resistance to cationic agents, which are important biocides, has been described in antibiotic-resistant Staphylococcus aureus. ...
    • Trends in therapeutic and prevention strategies for management of bovine mastitis: An overview
      Tiwari, J.G.; Babra, C.; Tiwari, H.; Williams, Vincent; De Wet, S.; Gibson, J.; Paxman, Adrian; Morgan, Eleanor; Costantino, Paul; Sunagar, Raju; Isloor, Shrikrishna; Mukkur, Trilochan (2013)
      Mastitis is one of the most economically significant diseases for the dairy industry for backyard farmers in developing countries and high producing herds worldwide. Two of the major factors impeding reduction in the ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.