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dc.contributor.authorNumnuam, A.
dc.contributor.authorChumbimuni-Torres, K.
dc.contributor.authorXiang, Y.
dc.contributor.authorBash, R.
dc.contributor.authorThavarungkul, P.
dc.contributor.authorKanatharana, P.
dc.contributor.authorPretsch, E.
dc.contributor.authorWang, J.
dc.contributor.authorBakker, Eric
dc.identifier.citationNumnuam, Apon and Chumbimuni-Torres, Karin Y. and Xiang, Yun and Bash, Ralph and Thavarungkul, Panote and Kanatharana, Proespichaya and Pretsch, Ern and Wang, Joseph and Bakker, Eric. 2008. Aptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes. Analytical Chemistry 80 (3): 707-712.

We here report on the first example of an aptamer-based potentiometric sandwich assay of proteins. The measurements are based on CdS quantum dot labels of the secondary aptamer, which were determined with a novel solid-contact Cd2+-selective polymer membrane electrode after dissolution with hydrogen peroxide. The electrode exhibited cadmium ion detection limits of 100 pM in 100 mL samples and of 1 nM in 200 microwells, using a calcium-selective electrode as a pseudoreference electrode. As a prototype example, thrombin was measured in 200 samples with a lower detection limit of 0.14 nM corresponding to 28 fmol of analyte. The results show great promise for the potentiometric determination of proteins at very low concentrations in microliter samples.

dc.publisherAmerican Chemical Society
dc.titleAptamer-Based Potentiometric Measurements of Proteins Using Ion-Selective Microelectrodes
dc.typeJournal Article
dcterms.source.titleAnalytical Chemistry
curtin.departmentNanochemistry Research Institute (Research Institute)
curtin.accessStatusFulltext not available
curtin.facultyNanochemistry Research Centre

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