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    Ion-Transfer Voltammetric Behavior of Protein Digests at Liquid|Liquid Interfaces

    Access Status
    Fulltext not available
    Authors
    Herzog, G.
    Roger, A.
    Sheehan, D.
    Arrigan, Damien
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Herzog, Gregoire and Roger, Amandine and Sheehan, David and Arrigan, Damien. 2010. Ion-Transfer Voltammetric Behavior of Protein Digests at Liquid|Liquid Interfaces. Analytical Chemistry. 82: pp. 258-264.
    Source Title
    Analytical Chemistry
    DOI
    10.1021/ac901909j
    ISSN
    00032700
    Faculty
    Nanochemistry Research Institute (NRI)
    Faculty of Science and Engineering
    School
    Nanochemistry Research Institute (Research Institute)
    URI
    http://hdl.handle.net/20.500.11937/11292
    Collection
    • Curtin Research Publications
    Abstract

    The development of new methods for the detection of proteins and peptides is of widespread importance. In this work, the electrochemical behavior of peptide mixtures resulting from proteolytic digestion of proteins was investigated at the polarized liquid|liquid interface (or the interface between two immiscible electrolyte solutions, ITIES). The influence of pepsin digestion on three proteins (hemoglobin, lysozyme, and cytochrome c) was studied, and it was revealed that resulting cyclic voltammograms of the three protein digests were different due to the unique peptide mixtures for a given protein. Differential pulse stripping voltammetry of protein digests enabled the detection of digested proteins at concentrations ranging between 0.55 and 4.22 M. A limit of detection of 0.55 M of the initial concentration of protein was achieved, demonstrating the analytical possibilities of such an electrochemical method. These results show that ion transfer voltammetry offers the opportunity to study and develop label-free detection of peptides resulting from enzymatic digestions of proteins and may thus have a role in development of new proteomic technologies.

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