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    Isolation and Detection of Ingested DNA From the Immature Stages of Calliphora dubia (Diptera: Calliphoridae): A Forensically Important Blowfly

    Access Status
    Fulltext not available
    Authors
    Carvalho, F.
    Dadour, I.
    Groth, David
    Harvey, M.
    Date
    2005
    Type
    Journal Article
    
    Metadata
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    Citation
    Carvalho, Filipa and Dadour, Ian and Groth, David and Harvey, Michelle. 2005. Isolation and Detection of Ingested DNA From the Immature Stages of Calliphora dubia (Diptera: Calliphoridae): A Forensically Important Blowfly. Forensic Science, Medicine and Pathology 1 (4): pp. 261-266.
    Source Title
    Forensic Science, Medicine and Pathology
    ISSN
    1547 769X
    Faculty
    Faculty of Health Sciences
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/11899
    Collection
    • Curtin Research Publications
    Abstract

    The forensic entomologist frequently bases time since death (TSD) estimation on fly larvae. In some cases, the food source on which these larvae have completed their development may be questionable, and requires verification to ensure the accuracy of the TSD estimation. Ingested DNA may be isolated from the alimentary canal of immature insects. Previous studies have confirmed the ability to extract ingested DNA from the alimentary tract of third instar blowfly larvae. This study considers the potential to detect ingested DNA from immature stages of the bluebodied blowfly Calliphora dubia (Macquart) that had fed on sheep liver. Individuals from early first instar larvae through day 3 pupae were surface decontaminated, followed by DNA isolation and detection by amplifying the sheep satellite I region. Fragments of 197 basepairs (bp) and 87 bp were successfully isolated and detected in all stages of immatures until 2-day-old pupae, with detection at this stage being unsuccessful on 3-day-old pupae. This study presents a suitable protocol for the isolation and detection of ingested DNA from immature stages of C. dubia.

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