Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens
|dc.identifier.citation||Goulter, R. and Taran, E. and Gentle, I. and Gobius, K. and Dykes, G. 2014. Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens. Colloids and Surfaces B: Biointerfaces. 119: pp. 90-98.|
The role of Escherichia coli H antigens in hydrophobicity and attachment to glass, Teflon and stainless steel (SS) surfaces was investigated through construction of fliC knockout mutants in E. coli O157:H7, O1:H7 and O157:H12. Loss of FliCH12 in E. coli O157:H12 decreased attachment to glass, Teflon and stainless steel surfaces (p < 0.05). Complementing E. coli O157:H12 ΔfliCH12 with cloned wildtype (wt) fliCH12 restored attachment to wt levels. The loss of FliCH7 in E. coli O157:H7 and O1:H7 did not always alter attachment (p > 0.05), but complementation with cloned fliCH12, as opposed to cloned fliCH7, significantly increased attachment for both strains compared with wt counterparts (p < 0.05). Hydrophobicity determined using bacterial adherence to hydrocarbons and contact angle measurements differed with fliC expression but was not correlated to the attachment to materials included in this study. Purified FliC was used to functionalise silicone nitride atomic force microscopy probes, which were used to measure adhesion forces between FliC and substrates. Although no significant difference in adhesion force was observed between FliCH12 and FliCH7 probes, differences in force curves suggest different mechanism of attachment for FliCH12 compared with FliCH7. These results indicate that E. coli strains expressing flagellar H12 antigens have an increased ability to attach to certain abiotic surfaces compared with E. coli strains expressing H7 antigens.
|dc.title||Escherichia coli strains expressing H12 antigens demonstrate an increased ability to attach to abiotic surfaces as compared with E. coli strains expressing H7 antigens|
|dcterms.source.title||Colloids and Surfaces B: Biointerfaces|
|curtin.department||School of Public Health|
|curtin.accessStatus||Fulltext not available|
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