Target DNA recognition using electrochemical impedance spectroscopy

Michaels, P.; Ciampi, Simone; Yean, C.; Gooding, J.

doi:10.1109/ICONN.2010.6045241

Access Status

Fulltext not available

Authors

Michaels, P.

Ciampi, Simone

Yean, C.

Gooding, J.

Date

2010

Type

Conference Paper

Metadata

Show full item record

Citation

Michaels, P. and Ciampi, S. and Yean, C. and Gooding, J. 2010. Target DNA recognition using electrochemical impedance spectroscopy, pp. 282-284.

Source Title

ICONN 2010 - Proceedings of the 2010 International Conference on Nanoscience and Nanotechnology

DOI

10.1109/ICONN.2010.6045241

ISBN

9781424452620

School

Nanochemistry Research Institute

URI

http://hdl.handle.net/20.500.11937/12630

Collection

Curtin Research Publications

Abstract

Electrochemical impedance spectroscopy was used to differentiate between different target DNA sequences without the use of a redox label or application of large bias voltages. This method relies on the formation of a mixed self-assembled monolayer (SAMs) composed of single-stranded DNA and a diluent, mercaptoethanol. Recognition of the DNA analytes was achieved through the significant increase in conductance for the double stranded DNA, as compared to single-stranded DNA. Non-complementary and single base-pair mismatches were also able to be differentiated. © 2010 IEEE.