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    Proteomic identification of extracellular proteins regulated by the Gna1 Gα subunit in Stagonospora nodorum

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    Access Status
    Open access
    Authors
    Tan, Kar-Chun
    Heazlewood, J.
    Millar, A.
    Oliver, Richard
    Solomon, P.
    Date
    2009
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Tan, Kar-Chun and Heazlewood, Joshua L. and Millar, Harvey A. and Oliver, Richard P. and Solomon, Peter S. 2009. Proteomic identification of extracellular proteins regulated by the Gna1 Gα subunit in Stagonospora nodorum. Mycological Research. 113 (5): pp. 523-531.
    Source Title
    Mycological Research
    DOI
    10.1016/j.mycres.2009.01.004
    ISSN
    0953-7562
    School
    Department of Environment and Agriculture
    Remarks

    NOTICE: This is the author’s version of a work that was accepted for publication in Journal of Mycological Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Mycological Research [113, 5, 2009] DOI 10.1016/j.mycres.2009.01.004

    URI
    http://hdl.handle.net/20.500.11937/13082
    Collection
    • Curtin Research Publications
    Abstract

    The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn.leaf and glume blotch) disease of wheat. The Gna1-encoded Ga protein is an important signaltransduction component in the fungus, which is required for full pathogenicity, sporulationand extracellular depolymerase production. In this study, we sought to gaina better understanding of defects associated with the gna1 mutant by using twodimensionalgel electrophoresis to analyse the extracellular proteome for differences tothe wildtype. Mass spectrometry analysis of altered abundant protein spots and peptidematching to the Stagonospora nodorum genome database have led to the identification ofgenes implicated in cell wall degradation, proteolysis, RNA hydrolysis and aromatic compoundmetabolism. In addition, quantitative RT-PCR has demonstrated that some of theencoding genes showed differential expression throughout host infection. Implicationsof these proteins and their corresponding genes in fungal virulence are discussed.The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn. leaf and glume blotch) disease of wheat. The Gna1-encoded Gα protein is an important signal transduction component in the fungus, which is required for full pathogenicity, sporulation and extracellular depolymerase production. In this study, we sought to gain a better understanding of defects associated with the gna1 mutant by using two-dimensional gel electrophoresis to analyse the extracellular proteome for differences to the wildtype. Mass spectrometry analysis of altered abundant protein spots and peptide matching to the Stagonospora nodorum genome database have led to the identification of genes implicated in cell wall degradation, proteolysis, RNA hydrolysis and aromatic compound metabolism. In addition, quantitative RT-PCR has demonstrated that some of the encoding genes showed differential expression throughout host infection. Implications of these proteins and their corresponding genes in fungal virulence are discussed.

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    • Proteomic identification of extracellular proteins regulated by the Gna1 G-alpha subunit in Stagonospora nodorum
      Tan, Kar-Chun; Heazlewood, J.; Millar, A.; Oliver, Richard; Solomon, P. (2009)
      The fungus Stagonospora nodorum is the causal agent of stagonospora nodorum blotch (syn. leaf and glume blotch) disease of wheat. The Gna1-encoded Gα protein is an important signal transduction component in the fungus, ...
    • A signalling-regulated short-chain dehydrogenase of Stagonospora nodorum regulates asexual development
      Tan, Kar-Chun; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
    • A Signaling-Regulated, Short-Chain Dehydrogenase of Stagonospora nodorum Regulates Asexual Development
      Tan, K.; Heazlewood, J.; Millar, A.; Thomson, G.; Oliver, Richard; Solomon, P. (2008)
      The fungus Stagonospora nodorum is a causal agent of leaf and glume blotch disease of wheat. It has been previously shown that inactivation of heterotrimeric G protein signaling in Stagonospora nodorum caused development ...
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