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    Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi

    Access Status
    Fulltext not available
    Authors
    Roberts, I.
    Oliver, Richard
    Punt, P.
    Van Den Hondel, C.
    Date
    1989
    Type
    Journal Article
    
    Metadata
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    Citation
    ROBERTS IN, OLIVER RP, PUNT PJ & VAN DEN HONDEL CAMJJ (1989) Expression of the E.coli ß-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Current Genetics 15 177-180
    DOI
    10.1007/BF00435503
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/13483
    Collection
    • Curtin Research Publications
    Abstract

    A chimaeric β-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Cotransformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke)) resulted in the expression of β-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi.

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