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    NaCS-PDMDAAC immobilized cultivation of recombinant Dictyostelium discoideum for soluble human Fas ligand production

    Access Status
    Fulltext not available
    Authors
    Zheng, C.
    Zeng, X.
    Danquah, Michael
    Lu, Y.
    Date
    2015
    Collection
    • Curtin Research Publications
    Type
    Journal Article
    Metadata
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    Abstract

    Background: Effective bimolecular adsorption of proteins onto solid matrices is characterized by in-depth understanding of the biophysical features essential to optimize the adsorption performance. Results: The adsorption of bovine serum albumin (BSA) onto anion-exchange Q-sepharose solid particulate support was investigated in batch adsorption experiments. Adsorption kinetics and isotherms were developed as a function of key industrially relevant parameters such as polymer loading, stirring speed, buffer pH, protein concentration and the state of protein dispersion (solid/aqueous) in order to optimize binding performance and adsorption capacity. Experimental results showed that the first order rate constant is higher at higher stirring speed, higher polymer loading, and under alkaline conditions, with a corresponding increase in equilibrium adsorption capacity. Increasing the stirring speed and using aqueous dispersion protein system increased the adsorption rate, but the maximum protein adsorption was unaffected. Regardless of the stirring speed, the adsorption capacity of the polymer was 2.8 mg/ml. However, doubling the polymer loading increased the adsorption capacity to 9.4 mg/ml. Conclusions: The result demonstrates that there exists a minimum amount of polymer loading required to achieve maximum protein adsorption capacity under specific process conditions.

    Citation
    Zheng, C. and Zeng, X. and Danquah, M. and Lu, Y. 2015. NaCS-PDMDAAC immobilized cultivation of recombinant Dictyostelium discoideum for soluble human Fas ligand production. Biotechnology Progress. 31 (2): pp. 424-430.
    Source Title
    Biotechnology Progress
    URI
    http://hdl.handle.net/20.500.11937/14161
    DOI
    10.1002/btpr.2032
    Department
    Curtin Sarawak

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