Extensive macrosynteny between Medicago truncatula and Lens culinaris ssp. Culinaris
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The first predominantly gene-based genetic linkage map of lentil (Lens culinaris ssp. culinaris) was constructed using an F5 population developed from a cross between the cultivars Digger (ILL5722) and Northfield (ILL5588) using 79 intron-targeted amplified polymorphic (ITAP) and 18 genomic simple sequence repeat (SSR) markers. Linkage analysis revealed seven linkage groups (LGs) comprised of 5–25 markers that varied in length from 80.2 to 274.6 cM. The genome map spanned a total length of 928.4 cM. Clear evidence of a simple and direct macrosyntenic relationship between lentil and Medicago truncatula was observed. Sixty-six out of the 71 gene-based markers, which were previously assigned to M. truncatula genetic and physical maps, were found in regions syntenic between the Lens c. ssp. culinaris and M. truncatula genomes. However, there was evidence of moderate chromosomal rearrangements which may account for the difference in chromosome numbers between these two legume species. Eighteen common SSR markers were used to connect the current map with the most comprehensive and recent map that exists for lentil, providing the syntenic context of four important domestication traits. The composite map presented, anchored with orthologous markers mapped in M. truncatula, provides a strong foundation for the future use of genomic and genetic information in lentil genetic analysis and breeding.
A copy of this item may be available from Professor Richard Oliver
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Construction of a comparative genetic map in faba bean (Vicia faba L.); conservation of genome structure with Lens culinarisEllwood, Simon; Phan, Huyen T.T.; Jordan, Megan; Hane, James; Torres, Anna M.; Avila, Carmen M.; Cruz-Izquierdo, Serafin; Oliver, Richard P. (2008)Background: The development of genetic markers is complex and costly in species with little pre-existing genomic information. Faba bean possesses one of the largest and least studied genomes among cultivated crop plants ...
Construction of a comparative genetic map in faba bean (Vicia faba L.); conservation of genome structure with Lens culinarisEllwood, Simon; Phan, H.; Jordan, M.; Hane, James; Torres, A.; Avila, C.; Cruz-Izquierdo, S.; Oliver, Richard (2008)Background: The development of genetic markers is complex and costly in species with little preexisting genomic information. Faba bean possesses one of the largest and least studied genomes among cultivated crop plants ...
Differences in syntenic complexity between Medicago truncatula with Lens culinaris and Lupinus albusPhan, H.; Ellwood, Simon; Ford, R.; Thomas, S.; Oliver, Richard (2006)Orthologous markers transferable between distantly related legume species allow for the rapid generation of genetic maps in species where there is little pre-existing genomic or EST information. We are using the model ...