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    Alcohol Oxidase is a Novel Pathogenicity Factor for Cladosporium fulvum but Aldehyde Dehydrogenase is Dispensable

    Access Status
    Fulltext not available
    Authors
    Segers, G.
    Bradshaw, N.
    Archer, D.
    Blissett, K.
    Oliver, Richard
    Date
    2001
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    SEGERS G, BRADSHAW N, ARCHER D, BLISSETT K, OLIVER RP (2001) Alcohol Oxidase is a Novel Pathogenicity Factor for Cladosporium fulvum but Aldehyde Dehydrogenase is Dispensable Molecular Plant Microbe Interactions 14 367-377
    DOI
    10.1094/MPMI.2001.14.3.367
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/14858
    Collection
    • Curtin Research Publications
    Abstract

    Cladosporium fulvum is a mitosporic ascomycete pathogen of tomato. A study of fungal genes expressed during carbon starvation in vitro identified several genes that were up regulated during growth in planta. These included genes predicted to encode acetaldehyde dehydrogenase (Aldh1) and alcohol oxidase (Aox1). An Aldh1 deletion mutant was constructed. This mutant lacked all detectable ALDH activity, had lost the ability to grow with ethanol as a carbon source, but was unaffected in pathogenicity. Aox1 expression was induced by carbon starvation and during the later stages of infection. The alcohol oxidase enzyme activity has broadly similar properties (Km values, substrate specificity, pH, and heat stability) to yeast enzymes. Antibodies raised to Hansenula polymorpha alcohol oxi-dase (AOX) detected antigens in Western blots of starved C. fulvum mycelium and infected plant material. Antigen reacting with the antibodies was localized to organelles resembling peroxisomes in starved mycelium and infected plants. Disruption mutants of Aox1 lacked detectable AOX activity and had markedly reduced pathogenicity as assayed by two different measures of fungal growth. These results identify alcohol oxidase as a novel pathogenicity factor and are discussed in relation to peroxisomal metabolism of fungal pathogens during growth in planta.

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