Alcohol Oxidase is a Novel Pathogenicity Factor for Cladosporium fulvum but Aldehyde Dehydrogenase is Dispensable
MetadataShow full item record
A copy of this item may be available from Professor Richard Oliver
Cladosporium fulvum is a mitosporic ascomycete pathogen of tomato. A study of fungal genes expressed during carbon starvation in vitro identified several genes that were up regulated during growth in planta. These included genes predicted to encode acetaldehyde dehydrogenase (Aldh1) and alcohol oxidase (Aox1). An Aldh1 deletion mutant was constructed. This mutant lacked all detectable ALDH activity, had lost the ability to grow with ethanol as a carbon source, but was unaffected in pathogenicity. Aox1 expression was induced by carbon starvation and during the later stages of infection. The alcohol oxidase enzyme activity has broadly similar properties (Km values, substrate specificity, pH, and heat stability) to yeast enzymes. Antibodies raised to Hansenula polymorpha alcohol oxi-dase (AOX) detected antigens in Western blots of starved C. fulvum mycelium and infected plant material. Antigen reacting with the antibodies was localized to organelles resembling peroxisomes in starved mycelium and infected plants. Disruption mutants of Aox1 lacked detectable AOX activity and had markedly reduced pathogenicity as assayed by two different measures of fungal growth. These results identify alcohol oxidase as a novel pathogenicity factor and are discussed in relation to peroxisomal metabolism of fungal pathogens during growth in planta.
Showing items related by title, author, creator and subject.
Reactive oxygen species level defines two functionally distinctive stages of inflammatory dendritic cell development from mouse bone marrowSheng, K.; Pietersz, G.; Tang, C.; Ramsland, Paul; Apostolopoulos, V. (2010)Reactive oxygen species (ROS) have been implicated in various physiological activities. However, their role in dendritic cell (DC) activation and generation has not been investigated. Using the bone marrow-derived ...
Genetic and Genomic Analysis of Rhizoctonia solani Interactions with Arabidopsis; Evidence of Resistance Mediated through NADPH OxidasesFoley, R.; Gleason, C.; Anderson, J.; Hamann, T.; Singh, Karambir (2013)Rhizoctonia solani is an important soil-borne necrotrophic fungal pathogen, with a broad host range and little effective resistance in crop plants. Arabidopsis is resistant to R. solani AG8 but susceptible to R. solani ...
Schwarz, Karen Rosemary (2012)A research project was undertaken to study the effect of biosolids on the decay times of enteric pathogens in the soil. This is the most comprehensive study in Australia where the persistence of enteric microorganisms in ...