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dc.contributor.authorO'Sullivan, S.
dc.contributor.authorArrigan, Damien
dc.date.accessioned2017-01-30T11:51:58Z
dc.date.available2017-01-30T11:51:58Z
dc.date.created2013-02-24T20:00:20Z
dc.date.issued2013
dc.identifier.citationO'Sullivan, Shane and Arrigan, Damien. 2013. Impact of a surfactant on the electroactivity of proteins at an aqueous-organogel microinterface array. Analytical Chemistry. 85 (3): pp. 1389-1394.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/15801
dc.identifier.doi10.1021/ac302222u
dc.description.abstract

The impact of surfactant addition to the organic phase on the electroactivity of proteins at the aqueous-organogel interface was examined by voltammetry. The presence of bis(2-ethylhexyl)sulfosuccinate (AOT) in the organogel phase, as the sodium salt, caused marked changes in the peak currents for myoglobin detection. The protein desorption voltammetric peak exhibited a 6-fold increase in the current compared to the corresponding experiment without surfactant. Interfacial coverage showed a 17-fold increase in the adsorbed protein at the interface, from 50 pmol cm-2, in the absence of surfactant, to 850 pmol cm-2, in the presence of 10 mM surfactant. Additionally, the presence of the surfactant resulted in a second pair of adsorption/desorption peaks at lower potentials and in a change in the capacitance of the system. The formation of surfactant-protein and surfactant-protein-organic anion deposits is proposed on the basis of these features, leading to increased voltammetric signals for myoglobin, hemoglobin, and cytochrome c. The mechanism of protein-surfactant interaction was probed by using the surfactant as the anion in the organic phase electrolyte salt. Repetitive cyclic voltammetry of cytochrome c showed that in the presence of surfactant there was an enhancement of the signal, caused by a build-up of the protein-surfactant-electrolyte anion assembly at the interface. These findings provide the basis for surfactant-modified interfaces to enhance the electroanalytical performance for protein detection.

dc.publisherAmerican Chemical Society
dc.titleImpact of a surfactant on the electroactivity of proteins at an aqueous-organogel microinterface array
dc.typeJournal Article
dcterms.source.volume85
dcterms.source.startPage1389
dcterms.source.endPage1394
dcterms.source.issn0003-2700
dcterms.source.titleAnalytical Chemistry
curtin.department
curtin.accessStatusFulltext not available


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