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    Detection of BK virus in urine from renal transplant subjects by mass spectrometry

    Access Status
    Open access via publisher
    Authors
    Konietzny, R.
    Fischer, R.
    Ternette, N.
    Wright, C.
    Turney, B.
    Chakera, Aron
    Hughes, D.
    Kessler, B.
    Pugh, C.
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Konietzny, R. and Fischer, R. and Ternette, N. and Wright, C. and Turney, B. and Chakera, A. and Hughes, D. et al. 2012. Detection of BK virus in urine from renal transplant subjects by mass spectrometry. Clinical Proteomics. 9 (1).
    Source Title
    Clinical Proteomics
    DOI
    10.1186/1559-0275-9-4
    ISSN
    1542-6416
    School
    Curtin Medical School
    URI
    http://hdl.handle.net/20.500.11937/16028
    Collection
    • Curtin Research Publications
    Abstract

    Background: The diagnosis and management of BK virus (BKV) reactivation following renal transplantation continues to be a significant clinical problem. Following reactivation of latent virus, impaired cellular immunity enables sustained viral replication to occur in urothelial cells, which potentially leads to the development of BKVassociated nephropathy (BKVAN). Current guidelines recommend regular surveillance for BKV reactivation through the detection of infected urothelial cells in urine (decoy cells) or viral nucleic acid in urine or blood. However, these methods have variable sensitivity and cannot routinely distinguish between different viral subtypes. We therefore asked whether mass spectrometry might be able to overcome these limitations and provide an additional noninvasive technique for the surveillance of BKV and identification of recipients at increased risk of BKVAN. Results: Here we describe a mass spectrometry (MS)-based method for the detection of BKV derived proteins directly isolated from clinical urine samples. Peptides detected by MS derived from Viral Protein 1 (VP1) allowed differentiation between subtypes I and IV. Using this approach, we observed an association between higher decoy cell numbers and the presence of the VP1 subtype Ib-2 in urine samples derived from a cohort of 20 renal transplant recipients, consistent with the hypothesis that certain viral subtypes may be associated with more severe BKVAN. Conclusions: This is the first study to identify BK virus proteins in clinical samples by MS and that this approach makes it possible to distinguish between different viral subtypes. Further studies are required to establish whether this information could lead to stratification of patients at risk of BKVAN, facilitate distinction between BKVAN and acute rejection (AR), and ultimately improve patient treatment and outcomes. © 2012 Konietzny et al.; licensee BioMed Central Ltd.

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