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    Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line: A Preliminary Study

    202621_134409_TB27-Poinern_BioMedResInt_2014.pdf (4.558Mb)
    Access Status
    Open access
    Authors
    Poinern, G.
    Le, X.
    O'Dea, M.
    Becker, Thomas
    Fawcett, D.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Poinern, G. and Le, X. and O'Dea, M. and Becker, T. and Fawcett, D. 2014. Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line: A Preliminary Study. BioMed Research International. 2014: 238762 (10 p.).
    Source Title
    BioMed Research International
    DOI
    10.1155/2014/238762
    ISSN
    2314-6133
    School
    Department of Applied Chemistry
    Remarks

    This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

    URI
    http://hdl.handle.net/20.500.11937/16306
    Collection
    • Curtin Research Publications
    Abstract

    In this preliminary study we investigate for the first time the biomedical potential of using porous anodic aluminium oxide (AAO) membranes as a cell substrate for culturing the Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line. One advantage of using the inorganic AAO membrane is the presence of nanometre scale pore channels that allow the exchangeof molecules and nutrients across the membrane. The size of the pore channels can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular interaction and response of theVero cell linewith an in-house synthesisedAAOmembrane, a commercially availablemembrane, and a glass controlwere assessed by investigating celladhesion, morphology, and proliferation over a 72 h period.The number of viable cells proliferating over the respective membrane surfaces revealed that the locally produced in-house AAO membrane had cells numbers similar to the glass control. The study revealed evidence of focal adhesion sites over the surface of the nanoporous membranes and the penetration of cellular extensions into the pore structure as well. The outcome of the study has revealed that nanometre scale porous AAO membranes have the potential to become practical cell culture scaffold substrates with the capability to enhance adhesion and proliferation of Vero cells.

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