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    AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass)

    Access Status
    Fulltext not available
    Authors
    Mucina, Ladislav
    Struwig, M.
    Mienie, C.
    van den Berg, J.
    Buys, M.
    Date
    2009
    Type
    Journal Article
    
    Metadata
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    Citation
    Struwig, M., Mienie, C.M.S., van den Berg, J., Mucina, L. & Buys, M.H. 2009. AFLPs are incompatible with RAPD and morphological data in Pennisetum purpureum (Napier grass). Bicohemical Systematics & Ecology 37: pp. 645-652.
    DOI
    10.1016/j.bse.2009.09.010
    Faculty
    Department of Environmental Biology
    Remarks

    Email: L.Mucina@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/17475
    Collection
    • Curtin Research Publications
    Abstract

    Accessions of Pennisetum purpureum in agricultural research centres in sub-Saharan Africa were genotyped using Amplified Fragment Length Polymorphisms (AFLPs) using primer combinations MluI/MseI. Cluster analyses of resulting bands suggested that the samples could be grouped into five large and five small-sized clusters. Principal coordinate analysis revealed two major clusters being well separated, with bulk of the samples forming one, poorly structured cluster. Our analyses failed to reveal the regional groupings found in previous studies using a subset of our sample universe and based on RAPDs as well as morphological and agronomical data. The inability of the AFLP data to clearly demarcate accessions is ascribed to either the presence of high levels of genetic uniformity, or nonoptimal primers and/or bad germplasm management practices. The re-registration of all accessions, based on DNA barcoding, is suggested as a means to resolve the lingering problems regarding the identity of accessions.

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