Candida albicans SOU1 encodes a sorbose reductase required for L-sorbose Utilization
dc.contributor.author | Greenberg, J. | |
dc.contributor.author | Price, N. | |
dc.contributor.author | Oliver, Richard | |
dc.contributor.author | Sherman, F. | |
dc.contributor.author | Rustchenko, E. | |
dc.date.accessioned | 2017-01-30T12:04:15Z | |
dc.date.available | 2017-01-30T12:04:15Z | |
dc.date.created | 2010-11-15T06:10:41Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Greenberg JR, Price NP, Oliver RP, Sherman F, & Rustchenko E. (2005) Candida albicans SOU1 encodes a sorbose reductase required for L-sorbose Utilization. Yeast. 22, 957-969 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/17830 | |
dc.identifier.doi | 10.1002/yea.1282 | |
dc.description.abstract |
Previous work in our laboratory showed that L-sorbose utilization in Candida albicans is subject to a novel form of regulation which involves a reversible increase or decrease in the copy number of chromosome 5. Furthermore, the structural gene SOU1 is required for L-sorbose utilization and encodes a member of the short chain dehydrogenase family. However, the precise function of SOU1 was not known and neither was the pathway for L-sorbose utilization. We have now expressed SOU1 at a high level from a replicative plasmid having a constitutive ADH1 promoter and purified a version of Sou1p tagged with the FLAG epitope at the N-terminus. Sou1FLAGNp has a sorbose reductase activity which utilizes NADPH as a co-factor and converts L-sorbose to D-sorbitol. It can also less efficiently utilize fructose as a substrate with NADPH as a co-factor, converting fructose to mannitol. In agreement with prediction, the purified enzyme has a subunit molecular weight of 31 kDa and a pI of about 4.8. It probably consists of four identical subunits and has a pH optimum of 6.2. The L-sorbose utilization pathway in C. albicans probably converts L-sorbose to fructose-6-phosphate via D-sorbitol as an intermediate. The first step is catalysed by Sou1p. We also found that C. albicans extracts have a D-sorbitol-6-phosphate dehydrogenase activity, not encoded by SOU1, which utilizes NADP as a co-factor. This activity has not been described previously in yeasts and may be involved in the conversion of phosphorylated D-sorbitol to fructose-6-phosphate or glucose-6-phosphate. | |
dc.title | Candida albicans SOU1 encodes a sorbose reductase required for L-sorbose Utilization | |
dc.type | Journal Article | |
curtin.note |
A copy of this item may be available from Professor Richard Oliver | |
curtin.note |
Email: Richard.oliver@curtin.edu.au | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Department of Environmental & Agriculture | |
curtin.faculty | School of Agriculture and Environment | |
curtin.faculty | Faculty of Science and Engineering |