An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA
dc.contributor.author | Ongkudon, C. | |
dc.contributor.author | Pan, S. | |
dc.contributor.author | Danquah, Michael | |
dc.date.accessioned | 2017-01-30T12:05:54Z | |
dc.date.available | 2017-01-30T12:05:54Z | |
dc.date.created | 2016-06-05T19:31:10Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Ongkudon, C. and Pan, S. and Danquah, M. 2013. An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA. Journal of Chromatography A. 1318: pp. 156-162. | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/18093 | |
dc.identifier.doi | 10.1016/j.chroma.2013.10.011 | |
dc.description.abstract |
The use of large DNAs in preparing multivalent vaccines that will eventually give protective immunity against multiple pathogenic microbes is becoming a major debate nowadays. One of the important issues in ensuring the successful implementation of the new vaccine technology is the development of a chromatographic technique that can handle larger DNAs. This paper reports the development of a novel conical monolithic column format with pore and surface characteristics engineered for the isolation of 25. kbp DNA in a single step fashion. An effective method of eliminating wall channelling, a defect of most conventional monolithic chromatography systems which has caused significant loss of product, was applied to maximise DNA recovery. This method was based on a systematic reduction of wall channel size based on a predetermined correlation between column's back pressure and wall channel size of a particular monolith pore size. | |
dc.publisher | Elsevier BV | |
dc.title | An innovative monolithic column preparation for the isolation of 25 kilo base pairs DNA | |
dc.type | Journal Article | |
dcterms.source.volume | 1318 | |
dcterms.source.startPage | 156 | |
dcterms.source.endPage | 162 | |
dcterms.source.issn | 0021-9673 | |
dcterms.source.title | Journal of Chromatography A | |
curtin.department | Curtin Sarawak | |
curtin.accessStatus | Fulltext not available |
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