Silk fibroin scaffolds with muscle-like elasticity support in vitro differentiation of human skeletal muscle cells
MetadataShow full item record
Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three-dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibroin scaffolds supported formation of long, well-aligned myotubes, whereas on Antheraea mylitta fibroin scaffolds the myotubes were thicker and shorter. Myotubes were oriented in two perpendicular layers on Antheraea assamensis scaffolds, and scaffolds of Philosamia/Samia ricini (S. ricini) fibroin poorly supported myotube formation. These differences were not caused by fibroin composition per se, as HSMMs adhered to, proliferated on and formed striated myotubes on all four fibroins presented as two-dimensional fibroin films. The Young's modulus of A. mylitta and B. mori scaffolds mimicked that of normal skeletal muscle, but A. assamensis and S. ricini scaffolds were more flexible. The present study demonstrates that although myoblasts deposit matrix onto fibroin scaffolds and create a permissive environment for cell proliferation, a scaffold elasticity resembling that of normal muscle is required for optimal myotube length, alignment, and maturation.
Showing items related by title, author, creator and subject.
Fabrication and Evaluation of Electrospun Silk Fibroin/Halloysite Nanotube Biomaterials for Soft Tissue RegenerationMohammadzadehmoghadam, Soheila; LeGrand, Catherine F; Dong, Yu; Wong, Chee-Wai; Kinnear, Beverley F; Dong, Roger ; Coombe, Deirdre R (2022)The production of nanofibrous materials for soft tissue repair that resemble extracellular matrices (ECMs) is challenging. Electrospinning uniquely produces scaffolds resembling the ultrastructure of natural ECMs. Herein, ...
Interactions between skeletal muscle myoblasts and their extracellular matrix revealed by a serum free culture systemChaturvedi, Vishal; Dye, Danielle; Kinnear, Bev; Van Kuppevelt, T.; Grounds, M.; Coombe, Deidre (2015)Decellularisation of skeletal muscle provides a system to study the interactions of myoblasts with muscle extracellular matrix (ECM). This study describes the efficient decellularisation of quadriceps muscle with the ...
The characterization of Abelson helper integration site-1 in skeletal muscle and its links to the metabolic syndromePrior, M.; Foletta, V.; Jowett, J.; Segal, D.; Carless, M.; Curran, J.; Dyer, T.; Moses, Eric; McAinch, A.; Konstantopoulos, N.; Bozaoglu, K.; Collier, G.; Cameron-Smith, D.; Blangero, J.; Walder, K. (2010)The human Abelson helper integration site-1 (AHI1) gene is associated with both neurologic and hematologic disorders; however, it is also located in a chromosomal region linked to metabolic syndrome phenotypes and was ...