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    Thorough assessment of DNA preservation from fossil bone and sediments excavated from a late Pleistocenee-Holocene cave deposit on Kangaroo Island, South Australia

    198004_198004.pdf (840.9Kb)
    Access Status
    Open access
    Authors
    Haouchar, D.
    Haile, James
    McDowell, M.
    Murray, D.
    White, Nicole
    Allcock, R.
    Phillips, M.
    Prideaux, G.
    Bunce, Michael
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Haouchar, Dalal and Haile, James and McDowell, Matthew C. and Murray, Daithi C. and White, Nicole E. and Allcock, Richard J.N. and Phillips, Matthew J. and Prideaux, Gavin J. and Bunce, Michael. 2014. Thorough assessment of DNA preservation from fossil bone and sediments excavated from a late Pleistocenee-Holocene cave deposit on Kangaroo Island, South Australia. Quaternary Science Reviews. 84: pp. 56-64.
    Source Title
    Quaternary Science Reviews
    DOI
    10.1016/j.quascirev.2013.11.007
    ISSN
    0277-3791
    Remarks

    NOTICE: This is the author’s version of a work that was accepted for publication in Quaternary Science Reviews. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Quaternary Science Reviews, Vol. 84 (2014). http://doi.org/10.1016/j.quascirev.2013.11.007

    URI
    http://hdl.handle.net/20.500.11937/18822
    Collection
    • Curtin Research Publications
    Abstract

    Fossils and sediments preserved in caves are an excellent source of information for investigating impacts of past environmental changes on biodiversity. Until recently studies have relied on morphology-based palaeontological approaches, but recent advances in molecular analytical methods offer excellent potential for extracting a greater array of biological information from these sites. This study presents a thorough assessment of DNA preservation from late Pleistocene-Holocene vertebrate fossils and sediments from Kelly Hill Cave Kangaroo Island, South Australia. Using a combination of extraction techniques and sequencing technologies, ancient DNA was characterised from over 70 bones and 20 sediment samples from 15 stratigraphic layers ranging in age from >20 ka to ~6.8 ka. A combination of primers targeting marsupial and placental mammals, reptiles and two universal plant primers were used to reveal genetic biodiversity for comparison with the mainland and with the morphological fossil record for Kelly Hill Cave. We demonstrate that Kelly Hill Cave has excellent long-term DNA preservation, back to at least 20 ka. This contrasts with the majority of Australian cave sites thus far explored for ancient DNA preservation, and highlights the great promise Kangaroo Island caves hold for yielding the hitherto-elusive DNA of extinct Australian Pleistocene species.

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