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    Applicability of bacterial biocementation in sustainable construction materials

    Access Status
    Fulltext not available
    Authors
    Dhami, Navdeep
    Mukherjee, Abhijit
    Reddy, M.
    Date
    2016
    Type
    Journal Article
    
    Metadata
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    Citation
    Dhami, N. and Mukherjee, A. and Reddy, M. 2016. Applicability of bacterial biocementation in sustainable construction materials. Asia-Pacific Journal of Chemical Engineering. [In Press].
    Source Title
    Asia-Pacific Journal of Chemical Engineering
    DOI
    10.1002/apj.2014
    ISSN
    1932-2135
    School
    Office of Research and Development
    URI
    http://hdl.handle.net/20.500.11937/19105
    Collection
    • Curtin Research Publications
    Abstract

    Urease production of calcifying bacterium, Bacillus megaterium SS3 was enhanced by using response surface methodology (RSM) in current study. The preliminary screening by Plackett–Burman's design revealed that among the tested factors, glucose, urea and NaHCO3 significantly (p < 0.05) enhanced the urease production. Further optimization of urease production by B. megaterium through RSM was achieved as 879 U mL−1 in the presence of glucose (21.7 g L−1), urea (21.1 g L−1) and NaHCO3 (1.9 g L−1) compared to 640 U mL−1 in unoptimized medium. The effect of different factors seems to promote the growth of bacterial isolate leading to improvement in urease production. As production of carbonates by ureolytic bacteria is dependent on urease enzyme, improvement in enzyme production enhanced the carbonate precipitation efficacy to 1.7-fold in optimized medium. Carbonic anhydrase production also increased from 120 U mL−1 to 190 U mL−1 in optimized medium.

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