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    A metabolomic approach to dissecting osmotic stress in the wheat pathogen Stagonospora nodorum

    Access Status
    Fulltext not available
    Authors
    Lowe, R.
    Lord, M.
    Rybak, K.
    Trengove, R.
    Oliver, Richard
    Solomon, P.
    Date
    2008
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    LOWE RG, LORD M, RYBAK K, TRENGOVE RD, OLIVER RP & SOLOMON PS (2008) A metabolomic approach to dissecting osmotic stress in the wheat pathogen Stagonospora nodorum. Fungal Genetics and Biology 45 1479-1486
    DOI
    10.1016/j.fgb.2008.08.006
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/20421
    Collection
    • Curtin Research Publications
    Abstract

    A non-targeted metabolomics approach was used to identify significant changes in metabolism upon exposure of the wheat pathogen Stagonospora nodorum to 0.5 M NaCl. The polyol arabitol, and to a lesser extent glycerol, was found to accumulate in response to the osmotic stress treatment. Amino acid synthesis was strongly down-regulated whilst mannitol levels were unaffected. A reverse genetic approach was undertaken to dissect the role of arabitol metabolism during salt stress. Strains of S. nodorum lacking a gene encoding an l-arabitol dehydrogenase (abd1), a xylitol dehydrogenase (xdh1) and a double-mutant lacking both genes (abd1xdh1) were exposed to salt and the intracellular metabolites analysed. Arabitol levels were significantly up-regulated upon salt stress in the xdh1 strains but were significantly lower than the wild-type. Arabitol was not significantly different in either the abd1 or the abd1xdh1 strains during osmotic stress but the concentration of glycerol was significantly higher indicating a compensatory mechanism in operation. Genome sequence analysis identified a second possible enzyme capable of synthesizing arabitol explaining the basal level of arabitol present in the abd1xdh1 strains. This study identified that arabitol is the primary compatible solute in S. nodorum but in-built levels of redundancy are present allowing the fungus to tolerate osmotic stress.

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      Gna1, a gene encoding a Gα subunit, a key component of signal transduction pathways, has been cloned and characterized in the wheat pathogen Stagonospora nodorum. Analysis of Gna1 expression during infection revealed a ...
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