Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis
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The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA sensitive wheat genotypes. The single copy ToxA gene was deleted from a wild-type race 1 P. tritici-repentis isolate via homologous recombination of a knockout construct. Expression of the ToxA transcript was found to be absent in transformants (toxa), as was ToxA protein production in fungal culture filtrates. Plant bioassays were conducted to test transformant pathogenicity. The toxa strains were unable to induce necrosis on ToxA sensitivity wheat genotypes. To our knowledge, this is the first demonstration of a targeted gene knockout in P. tritici-repentis. The ability to undertake gene deletions will facilitate the characterisation of other pathogenicity effectors of this economically significant necrotroph.
This is the accepted version of the following article: Moffat, C. and See, P.T. and Oliver, R. 2014. Generation of a ToxA knockout strain of the wheat tan spot pathogen Pyrenophora tritici-repentis. Molecular Plant Pathology. 15 (9): pp. 918-926., which has been published in final form at http://doi.org/10.1111/mpp.12154
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The emergence of tan spot disease caused by toxigenic Pyrenophora tritici-repentis in Australia is not associated with increased deployment of toxin-sensitive cultivarsOliver, Richard; Lord, M.; Rybak, K.; Faris, J.; Solomon, P. (2008)The wheat disease tan (or yellow leaf) spot, caused by Pyrenophora tritici-repentis, was first described in the period 1934 to 1941 in Canada, India, and the United States. It was first noted in Australia in 1953 and only ...
Moffat, Caroline; See, Pao Theen; Oliver, Richard (2014)The necrotrophic fungal pathogen Pyrenophora tritici-repentis causes tan spot, a major disease of wheat, throughout the world. The proteinaceous effector ToxA is responsible for foliar necrosis on ToxA-sensitive wheat ...
See, Pao Theen; Marathamuthu, K.; Iagallo, E.; Oliver, Richard; Moffat, Caroline (2018)The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) causes the major wheat disease tan spot, and produces multiple necrotrophic effectors that contribute to virulence. The proteinaceous effector ToxA induces ...