Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Erratum: Comparative toxicity of oleic and linoleic acid on human lymphocytes (Life Sciences (2006) 78 (1448-1456))

    Access Status
    Fulltext not available
    Authors
    Cury-Boaventura, M.
    Gorjao, R.
    De Lima, T.
    Newsholme, Philip
    Curi, R.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Cury-Boaventura, M. and Gorjao, R. and De Lima, T. and Newsholme, P. and Curi, R. 2014. Erratum: Comparative toxicity of oleic and linoleic acid on human lymphocytes (Life Sciences (2006) 78 (1448-1456)). Life Sciences. 112 (1-2).
    Source Title
    Life Sciences
    DOI
    10.1016/j.lfs.2005.07.038
    ISSN
    0024-3205
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/21549
    Collection
    • Curtin Research Publications
    Abstract

    Commercially available lipid emulsions for parenteral nutrition are mainly composed by long chain triacylglycerol containing a high proportion of linoleic acid (LA) or oleic acid (OA). The immunological impact of such therapy is particularly important because parenteral diets are often administered to critically ill patients as a mechanism to supply adequate nutrition during catabolic stress conditions. The comparative toxicity of OA and LA on human lymphocytes and the type of cell death induced by these fatty acids were determined in vitro. Parameters of cell death were investigated by flow cytometry-cell viability, DNA fragmentation, phosphatidylserine externalization, mitochondrial depolarization, neutral lipid accumulation and production of reactive oxygen species-and by fluorescence microscopy-chromatin condensation. Additionally a spectrofluorometric assay was employed to determine the activities of caspase-3, 6 and 8. Evidence is presented herein that OA is less toxic to human lymphocytes than LA. However, both fatty acids promoted apoptosis and necrosis of these cells. The mechanism of cell death induced by OA involved activation of caspase 3 while the mechanism of death induced by LA involved mitochondrial depolarization and ROS production. Importantly, neutral lipid accumulation may be a mechanism to protect lymphocytes against the toxicity induced by OA. OA may offer an immunological less problematic alternative to LA with respect to fatty acid composition of parenteral nutritional emulsions. © 2005 Elsevier Inc. All rights reserved.

    Related items

    Showing items related by title, author, creator and subject.

    • Activation of Survival and Apoptotic Signaling Pathways in Lymphocytes Exposed to Palmitic Acid
      Takahashi, H.; Cambiaghi, T.; Luchessi, A.; Hirabara, S.; Vinolo, M.; Newsholme, Philip; Curi, R. (2011)
      The toxicity of palmitic acid (PA) towards a human T-lymphocyte cell line (Jurkat) has been previously investigated but the mechanism(s) of PA action were unknown. In the current study, Jurkat cells were treated with ...
    • The “Trojan Horse” Approach to Tumor Immunotherapy: Targeting the Tumor Microenvironment
      Nelson, Delia; Fisher, S.; Robinson, B. (2014)
      Most anticancer therapies including immunotherapies are given systemically; yet therapies given directly into tumors may be more effective, particularly those that overcome natural suppressive factors in the tumor ...
    • Elevated levels of branched-chain amino acids have little effect on pancreatic islet cells, but L-arginine impairs function through activation of the endoplasmic reticulum stress response
      Mullooly, N.; Vernon, W.; Smith, D.; Newsholme, Philip (2014)
      New Findings - What is the central question of this study?: Recent studies have demonstrated strong correlations between circulating branched-chain amino acid (AA) levels and insulin resistance, a predictor of susceptibility ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.