Covalent labelling of the protochlorophyllide oxidoreductase from etioplast membranes with (3H)N-phenylmaleimide
dc.contributor.author | Oliver, Richard | |
dc.contributor.author | Griffiths, W. | |
dc.date.accessioned | 2017-01-30T12:26:49Z | |
dc.date.available | 2017-01-30T12:26:49Z | |
dc.date.created | 2010-11-12T02:04:10Z | |
dc.date.issued | 1981 | |
dc.identifier.citation | OLIVER RP & GRIFFITHS WT (1981) Covalent labelling of the protochlorophyllide oxidoreductase from etioplast membranes with (3H)N-phenylmaleimide. Biochemical Journal 195 93-101 | |
dc.identifier.uri | http://hdl.handle.net/20.500.11937/21703 | |
dc.description.abstract |
[3H]N-Phenylmaleimide has been used to covalently label in a specific manner the substrate-protected thiol groups of the enzyme protochlorophyllide reductase. In membrane preparations from oat (Avena sativa) and runner-bean (Phaseolus vulgaris) seedlings, two related peptides of mol.wts. 35000/37000 and 34000/35000 respectively and showing properties expected of the reductase have been identified, whereas the same technique with barley (Hordeum vulgare) extracts resulted in labelling a single peptide of mol.wt. 38000. Evidence is presented that both NADPH and protochlorophyllide are required for protection of the essential thiol groups on the reductase in oat extracts, a situation favouring a ternary complex as the structure for the photoactive enzyme--substrates intermediate. | |
dc.relation.uri | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1162858/?tool=pubmed | |
dc.title | Covalent labelling of the protochlorophyllide oxidoreductase from etioplast membranes with (3H)N-phenylmaleimide | |
dc.type | Journal Article | |
curtin.note |
A copy of this item may be available from Professor Richard Oliver | |
curtin.note |
Email: Richard.oliver@curtin.edu.au | |
curtin.accessStatus | Fulltext not available | |
curtin.faculty | Department of Environmental & Agriculture | |
curtin.faculty | School of Agriculture and Environment | |
curtin.faculty | Faculty of Science and Engineering |