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dc.contributor.authorVukovic, J.
dc.contributor.authorRuitenberg, M.
dc.contributor.authorRoet, K.
dc.contributor.authorFranssen, E.
dc.contributor.authorArulpragasam, Ajanthy
dc.contributor.authorSasaki, T.
dc.contributor.authorVerhaagen, J.
dc.contributor.authorHarvey, A.
dc.contributor.authorBusfield, S.
dc.contributor.authorPlant, G.
dc.date.accessioned2017-01-30T12:31:37Z
dc.date.available2017-01-30T12:31:37Z
dc.date.created2016-09-12T08:36:56Z
dc.date.issued2009
dc.identifier.citationVukovic, J. and Ruitenberg, M. and Roet, K. and Franssen, E. and Arulpragasam, A. and Sasaki, T. and Verhaagen, J. et al. 2009. The glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro. GLIA. 57 (4): pp. 424-443.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/22478
dc.identifier.doi10.1002/glia.20771
dc.description.abstract

The primary olfactory pathway in adult mammals has retained a remarkable potential for self-repair. A specialized glial cell within the olfactory nerve, called olfactory ensheathing cell (OEC), and their associated extracellular matrix are thought to play an important role during regenerative events in this system. To gain insight into novel molecules that could mediate the OEC-supported growth of axons within the olfactory nerve, gene expression profiling experiments were conducted which revealed high expression of the glycoprotein fibulin-3 in OECs. This observation was confirmed with quantitative PCR. In vivo, the distribution of all members of the fibulin family, fibulin-3 included, was localized to the lamina propria underneath the olfactory epithelium, in close association within olfactory nerve bundles. To manipulate fibulin-3 gene expression in cultured OECs, lentiviral vector constructs were designed to either transgenically express or knock-down fibulin-3. Experimental data showed that increased levels of fibulin-3 induced profound morphological changes in cultured OECs, impeded with their migratory abilities and also suppressed OEC-mediated neurite outgrowth. Knock-down of fibulin-3 levels resulted in reduced OEC proliferation. In conclusion, the data provide novel insights into a putative role for fibulin-3 in the regulation of cell migration and neurite outgrowth within the primary olfactory pathway. © 2008 Wiley-Liss, Inc.

dc.titleThe glycoprotein fibulin-3 regulates morphology and motility of olfactory ensheathing cells in vitro
dc.typeJournal Article
dcterms.source.volume57
dcterms.source.number4
dcterms.source.startPage424
dcterms.source.endPage443
dcterms.source.issn0894-1491
dcterms.source.titleGLIA
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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