Efficient isolation of lupin protein
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The increasing demand for low cost and non-genetically modified vegetable proteins has pushed the food industry to explore alternate sources of protein. Lupin has been found to have similar protein content and amino acid profile to that of soy. In particular, the Australian Sweet Lupin (Lupinus angustifolius), a low alkaloid variety of lupin, is high in protein and fibre and low in fat, making it an ideal food ingredient for health and well being. Concentrated form of lupin protein with protein content of 90% was prepared by alkaline extraction at pH 9.0 followed by acidic precipitation at eight different pH levels (4.0, 4.2, 4.4, 4.5, 4.6, 4.8, 5.0 and 5.5). The range of pH employed covered the isoelectric points of major legume proteins. The results revealed that there was no significant difference in protein content and yield of lupin protein isolates precipitated at pH 4.4, 4.5, 4.6, 4.8 and 5.0. The finding indicated that instead of using pH 4.5 for lupin protein precipitation, a higher pH such as 5.0 can be used that would result in decreased acid usage, thus providing a more economical approach to protein isolate production.
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The effects of lupin (lupinus angustifolius) protein isolation on its dietary fibre and whey proteinsCoorey, Ranil; Chao, K.; Kumar, Vivek; Jayasena, Vijay (2013)This study was conducted to evaluate the effects of protein isolation on dietary fibre and whey proteins of lupin flour. Commercially available flour was used in-order to understand the possible commercial applications ...
Jayasena, Vijay; Chih, Jun; Nasar-Abbas, S. (2011)The increasing demand for low cost and non-genetically modified vegetable proteins has pushed the food industry to explore alternative sources of protein. Lupin has similar protein content and amino acid profile to soy. ...
The effect of germination and food processing on the concentration and activity of bioactive compounds in Australian Sweet LupinRumiyati (2010)Lupin is a grain legume which is high in protein and fibre, but low in fat and starch. Lupin also contains bioactive compounds such as phenolic compounds. Many studies have shown that a diet containing lupin has health ...