Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing
MetadataShow full item record
Sequence-based methods for transcriptome characterization have typically relied on generation of either serial analysis of gene expression tags or expressed sequence tags. Although such approaches have the potential to enumerate transcripts by counting sequence tags derived from them, they typically do not robustly survey the majority of transcripts along their entire length. Here we show that massively parallel sequencing of randomly primed cDNAs, using a next-generation sequencing-by-synthesis technology, offers the potential to generate relative measures of mRNA and individual exon abundance while simultaneously profiling the prevalence of both annotated and novel exons and exon-splicing events. This technique identifies known single nucleotide polymorphisms (SNPs) as well as novel single-base variants. Analysis of these variants, and previously unannotated splicing events in the HeLa S3 cell line, reveals an overrepresentation of gene categories including those previously implicated in cancer.
Showing items related by title, author, creator and subject.
Wysocki, Beata J. (1999)One of the fundamental problems related to the development of direct sequence code division multiple access (DS CDMA) wireless data networks is design of spreading sequences possessing semi-optimal characteristics. In ...
The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clonesMoolhuijzen, Paula; Lew-Tabor, A.; Morgan, J.; Valle, M.; Peterson, D.; Dowd, S.; Guerrero, F.; Bellgard, M.; Appels, R. (2011)Background: Rhipicephalus (Boophilus) microplus (Rmi) a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete ...
Characterisation of australian MRSA strains ST75- and ST883-MRSA-IV and analysis of their accessory gene regulator locusMonecke, S.; Kanig, H.; Rudolph, W.; Müller, E.; Coombs, Geoffrey; Hotzel, H.; Slickers, P.; Ehricht, R. (2010)Background: Community-acquired methicillin-resistant Staphylococcus aureus have become a major problem in Australia. These strains have now been isolated throughout Australia including remote Indigenous communities that ...