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    Liquid and Thin-Layer Chromatography

    Access Status
    Fulltext not available
    Authors
    Lewis, Simon
    Lenehan, C.
    Date
    2013
    Type
    Book Chapter
    
    Metadata
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    Citation
    Lewis, S. W. and Lenehan, C. E. 2013. Liquid and Thin-Layer Chromatography, in Siegel, J. A. and Saukko, P. J. (ed), Encyclopedia of Forensic Sciences, 2nd ed, Vol.3, pp. 586-589. Waltham: Academic Press.
    Source Title
    Encyclopedia of Forensic Sciences
    DOI
    10.1016/B978-0-12-382165-2.00246-4
    URI
    http://hdl.handle.net/20.500.11937/23017
    Collection
    • Curtin Research Publications
    Abstract

    Liquid chromatography is a broad classification used to describe a variety of different chromatographic configurations that rely on the use of a liquid mobile phase. Paper, thin-layer, and classical column chromatography techniques, as well as high-performance liquid chromatography (HPLC) and ion chromatography, all belong to the class of liquid chromatography. These techniques are heavily used in forensic science to separate a wide range of analytes including, but not restricted to, illicit drugs, drugs, and drug metabolites in toxicology samples, explosives residues, and textile fiber dyes. A variety of separation mechanisms can be used within liquid chromatography; these include adsorption, ion-exchange, size-exclusion, affinity, and ion-pair formation. A discussion of these separation mechanisms can be found in Chromatography: Basic Principles. This article gives an overview of classical, high-performance, and thin-layer chromatographic techniques including experimental configurations and forensic applications.

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