Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Application of Plackett-Burman screening design to the modeling of grafted alginate-carrageenan beads for the immobilization of penicillin G acylase

    Access Status
    Fulltext not available
    Authors
    Elnashar, Magdy
    Wahba, M.
    Amin, M.
    Eldiwany, A.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Elnashar, M. and Wahba, M. and Amin, M. and Eldiwany, A. 2014. Application of Plackett-Burman screening design to the modeling of grafted alginate-carrageenan beads for the immobilization of penicillin G acylase. Journal of Applied Polymer Science. 131 (11).
    Source Title
    Journal of Applied Polymer Science
    DOI
    10.1002/app.40295
    ISSN
    0021-8995
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/23065
    Collection
    • Curtin Research Publications
    Abstract

    Grafted alginate-carrageenan beads were used to immobilize the industrial enzyme penicillin G acylase (PGA). Sixteen factors were screened with the Plackett-Burman design (PBD) to test their significance on the gel beads formation and enzyme immobilization process. The results of PBD showed a wide variation of 30-fold in the amount of immobilized penicillin G acylase (iPGA) from 11.9 to 354.16 U/g of beads; this reflected the importance of the optimizing process. Among the 16 tested factors, only 3 were proven to be significant. These factors were the enzyme buffer pH (N), enzyme soaking time (Q) with the gel beads, and enzyme concentration (P). The Pareto chart revealed that both Q and P exerted significant positive effects on the amount of iPGA, whereas N had a negative effect. We recommend further study to optimize only these three significant, distinctive enzyme factors. The PGA covalent attachment to the gel beads were proven by Fourier transform infrared spectroscopy, elemental analysis, and NaCl and reusability tests. The best gel bead formula succeeded in the immobilization of 354.16 U/g of beads and proved to be reusable 14 times, retaining 84% of the initial enzyme activity. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40295. Copyright © 2013 Wiley Periodicals, Inc.

    Related items

    Showing items related by title, author, creator and subject.

    • Pretreatment of wastewater containing fats and oils using an immobilized enzyme.
      Jia, Huanfei (2002)
      This thesis investigates an application of immobilized lipase for pre-treating wastewater containing fats and oils, which is difficult to treat practically. The kinetics of soluble lipase was studied for establishing ...
    • Novel carrier of grafted alginate for covalent immobilization of inulinase
      Elnashar, Magdy; Danial, E.; Awad, G. (2009)
      Inulinase has been extracted from Penicillium chrysogenum P36 and immobilized on a novel matrix of grafted biopolymer. The crude enzyme has been characterized in terms of specific activity, optimum temperature, and ...
    • Immobilization of halophilic Aspergillus awamori EM66 exochitinase on grafted k-carrageenan-alginate beads
      Esawy, M.; Awad, G.; Wahab, W.; Elnashar, Magdy; El-Diwany, A.; Easa, S.; El-beih, F. (2016)
      A novel extreme halophilic exochitinase enzyme was produced by honey isolate Aspergillus awamori EM66. The enzyme was immobilized successfully on k-carrageenan-alginate gel carrier (CA) with 93 % immobilization yield. The ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.