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    Antibody modified porous silicon microparticles for the selective capture of cells

    Access Status
    Fulltext not available
    Authors
    Guan, B.
    Magenau, A.
    Ciampi, Simone
    Gaus, K.
    Reece, P.
    Gooding, J.
    Date
    2014
    Type
    Journal Article
    
    Metadata
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    Citation
    Guan, B. and Magenau, A. and Ciampi, S. and Gaus, K. and Reece, P. and Gooding, J. 2014. Antibody modified porous silicon microparticles for the selective capture of cells. Bioconjugate Chemistry. 25 (7): pp. 1282-1289.
    Source Title
    Bioconjugate Chemistry
    DOI
    10.1021/bc500144u
    ISSN
    1043-1802
    School
    Nanochemistry Research Institute
    URI
    http://hdl.handle.net/20.500.11937/24036
    Collection
    • Curtin Research Publications
    Abstract

    Herein, the ability of porous silicon (PSi) particles for selectively binding to specific cells is investigated. PSi microparticles with a high reflectance band in the reflectivity profile are fabricated, and subsequently passivated and modified with antibodies via the Cu(I)-catalyzed alkyne-azide cycloaddition reaction and succimidyl activation. To demonstrate the ability of the antibody-modified PSi particles to selectively bind to one cell type over others, HeLa cells were transfected with surface epitopes fused to fluorescent proteins. The antibody-functionalized PSi particles showed good selectivity for the corresponding surface protein on HeLa cells, with no significant cross-reactivity. The results are important for the application of PSi particles in cell sensing and drug delivery.

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