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dc.contributor.authorBustam, B.
dc.contributor.authorDixon, Kingsley
dc.contributor.authorBunn, E.
dc.date.accessioned2017-01-30T12:41:10Z
dc.date.available2017-01-30T12:41:10Z
dc.date.created2015-06-10T20:00:53Z
dc.date.issued2014
dc.identifier.citationBustam, B. and Dixon, K. and Bunn, E. 2014. Proliferation and harvesting of secondary protocorms as a novel means for improving propagation of terrestrial orchids. Australian Journal of Botany. 62 (7): pp. 614-621.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/24130
dc.identifier.doi10.1071/BT14291
dc.description.abstract

This study investigated optimisation of media and primary-protocorm development stages to enhance secondary-protocorm production as a novel means for propagation of terrestrial orchids, including taxa of conservation concern. Seeds of Caladenia latifolia were germinated asymbiotically on ½-strength Murashige and Skoog (MS) medium fortified with 5% (v/v) coconut water. Resulting protocorms at 3, 5 and 7 weeks of growth were subcultured to protocorm-proliferation media treatments consisting of ½-strength MS basal-salts medium with 6-benzylaminopurine (BA) and α- naphthaleneacetic acid (NAA) singly or in combination. Conversion of seeds to primary protocorms was high (87–92%). The highest percentage of secondary-protocorm proliferation was 40.1%, using 5-week-old protocorms (early Stage 4 of protocorm development) as explants and cultured on ½-strength MS with a combination of 5 µM NAA + 2 µM BA. Half-strength MS media containing a single plant-growth regulator (BA or NAA) were substantially less effective (<10% protocorm proliferation). The present study has provided a novel approach to sequential protocorm production that will be of value particularly for threatened orchids with limited seed availability. Protocorm proliferation in vitro enables a renewable supply of protocorms with which to conduct propagation, cryostorage and pilot restoration programs.

dc.publisherCSIRO Publishing
dc.titleProliferation and harvesting of secondary protocorms as a novel means for improving propagation of terrestrial orchids
dc.typeJournal Article
dcterms.source.volume62
dcterms.source.number7
dcterms.source.startPage614
dcterms.source.endPage621
dcterms.source.issn00671924
dcterms.source.titleAustralian Journal of Botany
curtin.accessStatusFulltext not available


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