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    A transient expression system to assay putative antifungal genes on powdery mildew infected barley leaves

    Access Status
    Fulltext not available
    Authors
    Nielsen, K.
    Olsen, O.
    Oliver, Richard
    Date
    1999
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    NIELSEN K, OLSEN O & OLIVER R (1999) A transient expression system to assay putative antifungal genes on powdery mildew infected barley leaves. Physiological and Molecular Plant Pathology 54 1-12
    DOI
    10.1006/pmpp.1998.0184
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/24545
    Collection
    • Curtin Research Publications
    Abstract

    A transient expression system has been established to assay the regulation and impact of putative antifungal proteins at the single cell level in barley (Hordeum vulgareL.) epidermal leaf cells infected with powdery mildew (Erysiphe graminisf.sp.hordei).The system combines biolistic transformation with direct comparison of the fungal attack in transfected and wild type cells. Many epidermal cells showed detectable expression of marker genes including a novel improved version of green fluorescent protein (GFP). The marker was detectable for at least 10 days during which individual host cells supported an unaffected asexual life-cycle of the pathogen.

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