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    Influence of the semisynthetic bile acid MKC on the ileal permeation of gliclazide in vitro in healthy and diabetic rats treated with probiotics

    Access Status
    Fulltext not available
    Authors
    Al-Salami, Hani
    Butt, G.
    Tucker, I.
    Mikov, M.
    Date
    2008
    Type
    Journal Article
    
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    Citation
    Al-Salami, H. and Butt, G. and Tucker, I. and Mikov, M. 2008. Influence of the semisynthetic bile acid MKC on the ileal permeation of gliclazide in vitro in healthy and diabetic rats treated with probiotics. Methods and Findings in Experimental and Clinical Pharmacology. 30 (2): pp. 107-113.
    Source Title
    Methods and Findings in Experimental and Clinical Pharmacology
    DOI
    10.1358/mf.2008.30.2.1159652
    ISSN
    0379-0355
    School
    School of Pharmacy
    URI
    http://hdl.handle.net/20.500.11937/24675
    Collection
    • Curtin Research Publications
    Abstract

    The aim of this study was to investigate the influence of sodium 3a,7a-dihydroxy-12-keto-5ß-cholanate (MKC) on the ileal permeation of gliclazide in healthy and diabetic rats treated with probiotics. Male Wistar rats (2-3 months, 350 ± 50 g) were randomly allocated into four groups (n = 32); Groups 1 and 2 were healthy controls and Groups 3 and 4 were diabetic rats (alloxan 30 mg/kg was administered i.v.), which were administered probiotics for three days after the rats became diabetics. The rats were sacrificed and tissues were mounted on Ussing chambers. Then, gliclazide (200 µg/ml) was added to all the groups, while MKC (50 µg/ml) was given to Groups 2 and 4, for the measurement of the mucosal to serosal absorption Jss(MtoS) and serosal to mucosal secretion Jss(StoM) of gliclazide. In the tissues of healthy rats treated with probiotics, MKC stimulated the net absorption of gliclazide by stimulating the absorptive and reducing the secretory unidirectional fluxes, while in tissues from diabetic rats treated with probiotics, MKC had no effect. In healthy rats treated with probiotics, the degradation of MKC by bacterial polypeptides produced divalent bile salts that inhibited Mrp2, which resulted in reducing secretion and stimulating the absorption of gliclazide. In contrast, in diabetic rats treated with probiotics, MKC had no effect possibly due to a difference in the metabolic profile and resulting in no net flux. Copyright 2008 Prous Science, S.A.U. or its licensors. All rights reserved.

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