Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum
MetadataShow full item record
Protoplasts of a pAN7-1-transformed isolated of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed.
A copy of this item may be available from Professor Richard Oliver
Showing items related by title, author, creator and subject.
The complexity of Rhipicephalus (Boophilus) microplus genome characterised through detailed analysis of two BAC clonesMoolhuijzen, Paula; Lew-Tabor, A.; Morgan, J.; Valle, M.; Peterson, D.; Dowd, S.; Guerrero, F.; Bellgard, M.; Appels, R. (2011)Background: Rhipicephalus (Boophilus) microplus (Rmi) a major cattle ectoparasite and tick borne disease vector, impacts on animal welfare and industry productivity. In arthropod research there is an absence of a complete ...
van Leeuwen, Stephen J. (1997)An understanding of the role of genetic and ecological factors that influence demographic change is paramount for the conservation of plant populations. These genetic and ecological factors often act in concert to influence ...
Cowling, W.; Stefanova, Katia; Beeck, C.; Nelson, M.; Hargreaves, B.; Sass, O.; Gilmour, A.; Siddique, K. (2015)© 2015 Cowling et al. We used the animal model in S0 (F1) recurrent selection in a self-pollinating crop including, for the first time, phenotypic and relationship records from self progeny, in addition to cross progeny, ...