Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Preparation and characterization of poly(lactic-co-glycolic acid) microparticles containing DNA molecules encoding a malaria vaccine candidate

    Access Status
    Fulltext not available
    Authors
    Liu, S.
    Danquah, Michael
    Ho, J.
    Ma, C.
    Wang, L.
    Coppel, R.
    Forde, G.
    Date
    2009
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Liu, S. and Danquah, M. and Ho, J. and Ma, C. and Wang, L. and Coppel, R. and Forde, G. 2009. Preparation and characterization of poly(lactic-co-glycolic acid) microparticles containing DNA molecules encoding a malaria vaccine candidate. Journal of Chemical Technology and Biotechnology. 84 (5): pp. 782-788.
    Source Title
    Journal of Chemical Technology and Biotechnology
    DOI
    10.1002/jctb.2112
    ISSN
    0268-2575
    School
    Curtin Sarawak
    URI
    http://hdl.handle.net/20.500.11937/25048
    Collection
    • Curtin Research Publications
    Abstract

    Background: A novel ultrasonic atomization approach for the formulation of biodegradable poly(lactic-co-glycolic acid) (PLGA) microparticles of a malaria DNA vaccine is presented. A 40 kHz ultrasonic atomization device was used to create the microparticles from a feedstock containing 5 volumes of 0.5% w/v PLGA in acetone and 1 volume of condensed DNA which was fed at a flow rate of 18ml h-1. The plasmid DNA vectors encoding a malaria protein were condensed with a cationic polymer before atomization. Results: High levels of gene expression in vitro were observed in COS-7 cells transfected with condensed DNA at a nitrogen to phosphate (N/P) ratio of 10. At this N/P ratio, the condensed DNA exhibited a monodispersed nanoparticle size (Z-average diameter of 60.8 nm) and a highly positive zeta potential of 38.8mV. The microparticle formulations of malaria DNA vaccine were quality assessed and it was shown that themicroparticles displayed high encapsulation efficiencies between 82-96% and a narrow size distribution in the range of 0.8-1.9 µm. In vitro release profile revealed that approximately 82% of the DNA was released within 30 days via a predominantly diffusion controlledmass transfer system. Conclusions: This ultrasonic atomization technique showed excellent particle size reproducibility and displayed potential as an industrially viable approach for the formulation of controlled release particles. © 2009 Society of Chemical Industry.

    Related items

    Showing items related by title, author, creator and subject.

    • Microparticle-mediated gene delivery for the enhanced expression of a 19-KDa fragment of merozoite surface protein 1 of Plasmodium falciparum
      Liu, S.; Danquah, Michael; Forde, G.; Ma, C.; Wang, L.; Coppel, R. (2010)
      The 19 kDa carboxyl-terminal fragment of merozoite surface protein 1 (MSP119) is a major component of the invasion-inhibitory response in individual immunity to malaria. A novel ultrasonic atomization approach for the ...
    • Simple Route to Lattice Energies in the Presence of Complex Ions
      Glasser, Leslie (2012)
      Lattice energies for ionic materials which separate into independent gaseous ions can be calculated bystandard Born-Haber-Fajans thermochemical cycle procedures, based on the energies of formation of those ions. However, ...
    • Encapsulation of hydrocortisone and mesalazine in zein microparticles
      Lau, E.; Giddings, S.; Mohammed, S.; Dubois, Paul; Johnson, Stuart; Stanley, R.; Halley, P.; Steadman, K. (2013)
      Zein was investigated for use as an oral-drug delivery system by loading prednisolone into zein microparticles using coacervation. To investigate the adaptability of this method to other drugs, zein microparticles were ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.