Reactive amine surfaces for biosensor applications, prepared by plasma-enhanced chemical vapour modification of polyolefin materials
Access Status
Authors
Date
2010Type
Metadata
Show full item recordCitation
Source Title
ISSN
School
Remarks
NOTICE: this is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors and Bioelectronics, vol. 25, no. 8 (2010) http://dx.doi.org/10.1016/j.bios.2009.12.034
Collection
Abstract
Here we have demonstrated a solventless plasma-based process that integrates low-cost, high throughput,high reproducibility and ecofriendly process for the functionalization of the next-generationpoint-of-care device platforms. Amine functionalities were deposited by plasma-enhanced chemicalvapour deposition (PECVD) using a new precursor. The influence of the plasma RF power and the deposition time on surfacial properties, as well as their effect on the reactivity and content of amino groups was investigated. The key process determinants were to have a sufficient power in the plasma to activate and partially fragment the monomer but not too much as to lose the reactive amine functionality, and sufficient deposition time to develop a reactive layer but not to consume or erode the amine reactivity. An immunoassay performed using human immunoglobulin (IgG) as a model analyte showed an improvement of the detection limit by two orders of magnitude beyond that obtained using devices activated by liquid-phase reaction.
Related items
Showing items related by title, author, creator and subject.
-
Takechi, Ryusuke (2010)It has been reported that lifestyle including diet is associated with Alzheimer’s disease (AD) risk and progression. Population studies indicate that the chronic consumption of diets enriched in saturated fats (SFA) and ...
-
Takechi, Ryu; Alfonso, Helman; Hiramatsu, N.; Ishisaka, A.; Tanaka, A.; Tan, L.; Lee, Andy (2016)This study investigated green tea catechins in plasma and urine and chronic disease biomarkers. We hypothesized that plasma and urinary concentration of green tea catechins are associated with cardiovascular disease and ...
-
Pallebage-Gamarallage, Menuka Madhavi Somapala (2012)Alzheimer’s disease (AD) is the most common cause of dementia pathologically characterised by neurovascular inflammation, extracellular proteinaceous deposits enriched in amyloid-β (Aβ) and formation of neurofibrillar ...